Western Blot (WB)
(Western blot detection of EGFP/EYFP expression in CHO-K1 transfected by different EGFP-fusion proteins (MW:A. 30kDa, B. 38kDa, C. 50kDa) or non-transfected (B) cell lysates using EGFP/EYFP rabbit pAb (1:10000 diluted).)
Rabbit anti-Transfected GFP/eGFP-tag Polyclonal Antibody | anti-GFP/eGFP antibody
Anti-GFP/eGFP-tag Rabbit pAb
Reactivity
Transfected
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
GFP/eGFP-tag; Polyclonal Antibody; Anti-GFP/eGFP-tag Rabbit pAb; GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein; anti-GFP/eGFP antibody
Host
Rabbit
Reactivity
Transfected
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified rabbit polyclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Concentration
1.5mg/ml (varies by lot)
Sequence Length
265
Applicable Applications for anti-GFP/eGFP antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:10000
Immunogen
Purified recombinant EYFP.tag full length expressed in E Coli.
Sensitivity
Transfected
Antibody Type
Tag antibody
Preparation and Storage
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Western Blot (WB)
(Western blot detection of EGFP/EYFP expression in CHO-K1 transfected by different EGFP-fusion proteins (MW:A. 30kDa, B. 38kDa, C. 50kDa) or non-transfected (B) cell lysates using EGFP/EYFP rabbit pAb (1:10000 diluted).)
Western Blot (WB)
(Western blot detection of EGFP/EYFP expression in CHO-K1 transfected by different EGFP-fusion proteins (MW:A. 30kDa, B. 38kDa, C. 50kDa) or non-transfected (B) cell lysates using EGFP/EYFP rabbit pAb (1:10000 diluted).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of CHO-K1 cells lysates transfected with EGFP-Cyclin D2 fragment fusion protein. IP: EGFP/EYFP rabbit pAb; WB: Cyclin D2 mouse mAb (200500).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of CHO-K1 cells lysates transfected with EGFP-Cyclin D2 fragment fusion protein. IP: EGFP/EYFP rabbit pAb; WB: Cyclin D2 mouse mAb (200500).)
Related Product Information for anti-GFP/eGFP antibody
Background : Swiss-Prot Acc.C8CHS1.Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization.
Product Categories/Family for anti-GFP/eGFP antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GFP/eGFP egfp (Catalog #AAA475561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GFP/eGFP-tag Rabbit pAb reacts with Transfected and may cross-react with other species as described in the data sheet. AAA Biotech's GFP/eGFP-tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:10000. Researchers should empirically determine the suitability of the GFP/eGFP egfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP/eGFP-tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
