Western Blot (WB)
(Western blot analysis of cell extracts from porcine aortic endothelial cells (PAECs) 3 days postconfluence treated with 0.3mM H2O2 for 30 minutes or calf intestinal alkaline phosphatase (CIP), using MBS624591 (upper) or control eNOS antibody (lower).)
Rabbit anti-Human eNOS Polyclonal Antibody | anti-eNOS antibody
eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein A and peptide affinity chromatography.
Synonyms
eNOS; Polyclonal Antibody; phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase; NOS 3; NOS III); Anti -eNOS; anti-eNOS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of eNOS only when phosphorylated at threonine 495. Species Crossreactivity: Bovine and porcine. Reactivity is expected with human, mouse and rat due to 100% sequence homology with peptide used for immunogen.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-eNOS antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:1000
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr495 of human eNOS.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of cell extracts from porcine aortic endothelial cells (PAECs) 3 days postconfluence treated with 0.3mM H2O2 for 30 minutes or calf intestinal alkaline phosphatase (CIP), using MBS624591 (upper) or control eNOS antibody (lower).)
Western Blot (WB)
(Western blot analysis of cell extracts from porcine aortic endothelial cells (PAECs) 3 days postconfluence treated with 0.3mM H2O2 for 30 minutes or calf intestinal alkaline phosphatase (CIP), using MBS624591 (upper) or control eNOS antibody (lower).)
Related Product Information for anti-eNOS antibody
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). eNOS is regulated by phosphorylation at multiple sites. The two most throughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
Product Categories/Family for anti-eNOS antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The eNOS enos (Catalog #AAA624591) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eNOS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Immunoprecipitation and Western Blot. Dilution: Immunoprecipitation: 1:100 Western Blot: 1:1000. Researchers should empirically determine the suitability of the eNOS enos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
