Rabbit GSK3beta Polyclonal Antibody | anti-GSK3beta antibody

GSK3beta (Phospho-Ser9) Antibody

Cat. Num. AAA9412426

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: GSK3beta; Polyclonal Antibody; GSK3beta (Phospho-Ser9) Antibody; GSK3b (Phospho-Ser9) Antibody; Factor A; GSK-3 beta; Protein kinase GSK-3-beta; kinase GSK-3 beta; anti-GSK3beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 420

• Applicable Applications for anti-GSK3beta antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3beta.
• Modification: Phospho-Ser9
• Target Name: GSK3beta
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3beta(Phospho-Ser9) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3beta (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide #51002 (right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3beta(Phospho-Ser9) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3beta (Phospho-Ser9) Antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3beta (Phospho-Ser9) Antibody.)

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3beta (Phospho-Ser9) Antibody.)

Related Product Information for anti-GSK3beta antibody

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Product Categories/Family for anti-GSK3beta antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 225903437
• NCBI GeneID: 2932
• NCBI Accession #: NP_001139628.1
• NCBI GenBank Nucleotide #: NM_001146156.1
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: glycogen synthase kinase-3 beta isoform 2
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.11.26; Protein kinase, CMGC; Kinase, protein; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: centrosome; dendritic spine; ribonucleoprotein complex; beta-catenin destruction complex; cytosol; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: integrin binding; NF-kappaB binding; ionotropic glutamate receptor binding; protein serine/threonine kinase activity; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; tau protein binding; protein kinase binding; ATP binding

Biological Process: fat cell differentiation; circadian rhythm; glycogen metabolic process; axon guidance; negative regulation of MAP kinase activity; re-entry into mitotic cell cycle; genetic imprinting; nerve growth factor receptor signaling pathway; positive regulation of protein binding; positive regulation of axon extension; protein amino acid autophosphorylation; response to lithium ion; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; myoblast fusion; ER overload response; negative regulation of glycogen biosynthetic process; response to drug; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; peptidyl-serine phosphorylation; organ morphogenesis; negative regulation of neuron maturation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity