Rabbit GSK3beta Polyclonal Antibody | anti-GSK3beta antibody

GSK3beta (Phospho-Ser9)

Cat. Num. AAA191014

• Applications: Western Blot, Immunohistochemistry
• Synonyms: GSK3beta; Polyclonal Antibody; GSK3beta (Phospho-Ser9); anti-GSK3beta antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human, mouse, and rat GSK3 beta only when phosphorylated at serine 9.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 420

• Applicable Applications for anti-GSK3beta antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: use at dilution of 1:500- 1:1,000. A band of ~46kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3beta and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-GSK3beta antibody

GSK3beta participates in the Wnt signaling pathway and is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. GSK3beta phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. It also phosphorylates CTNNB1/beta-catenin and MUC1 in breast cancer cells and decreases the interaction of MUC1 with CTNNB1/beta-catenin.

NCBI and Uniprot Product Information

• NCBI GI #: 225903437
• NCBI GeneID: 2932
• NCBI Accession #: NP_001139628.1
• NCBI GenBank Nucleotide #: NM_001146156.1
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: glycogen synthase kinase-3 beta isoform 2
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: CMGC group; EC 2.7.11.1; EC 2.7.11.26; GSK family; GSK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: beta-catenin destruction complex; centrosome; cytoplasm; cytosol; nucleus; plasma membrane

Molecular Function: beta-catenin binding; kinase activity; NF-kappaB binding; p53 binding; protease binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity; ubiquitin protein ligase binding

Biological Process: circadian rhythm; epithelial to mesenchymal transition; ER overload response; glycogen metabolic process; hippocampus development; negative regulation of apoptosis; negative regulation of glycogen biosynthetic process; negative regulation of NFAT protein import into nucleus; negative regulation of protein binding; negative regulation of protein complex assembly; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of microtubule-based process; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin