Rabbit GRP78 Polyclonal Antibody | anti-GRP78 antibody

GRP78 Polyclonal Antibody

Cat. Num. AAA8501598

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Reactivity: Human, Mouse, Rat, Pig
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Purified by protein A column
• Synonyms: GRP78; Polyclonal Antibody; GRP78 Polyclonal Antibody; anti-GRP78 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Pig
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by protein A column
• Form/Format: Each vial at least contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of 0.01 M PBS, PH7.4, 1 mg/ml BSA, 20 % glycerol, 0.01 % (w/v) NaN3
• Sequence Length: 653

• Applicable Applications for anti-GRP78 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-5000; ICC: 1:50-250
• Immunogen: Recombinant human GRP78 (rh-GRP78), C-terminal of GRP78
• Preparation and Storage: Store at-20 degree C upon arrival for one year. Avoid repeated freeze-thaw cycles.

Testing Data

Testing Data #2

(IHC of human colon cancer tissue (Stage IIIA, x100)1st AB: E14-14, 1:2502nd AB: ChemMateTMEnVision+/HRPBrown: GRP78Blue: nuclear staining with hematoxylin)

Related Product Information for anti-GRP78 antibody

Glucose-regulated protein 78 (GRP78), also known as immunoglobulin heavy chain binding protein (BiP) or heat shock protein A5 (HSPA5), is one of the most important chaperons located on endoplasmic reticulum and a member of heat shock protein 70 family. GRP78 expresses conservatively among a wide variety of biological species, and acts as a central regulator in endoplasmic reticulum (ER) functions, involving in ER protein folding and assembly process, and maintaining ER Ca2+ homeostasis, unfolded protein response and specific anti-apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 386758
• NCBI GeneID: 3309
• NCBI Official Full Name: GRP78, partial
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 5
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: endoplasmic reticulum chaperone BiP
• UniProt Protein Name: Endoplasmic reticulum chaperone BiP
• Protein Family: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: HSP70 family protein 5Curated

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Uniprot Description

Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 ().