Mouse GRP78 Monoclonal Antibody | anti-GRP78 antibody

GRP78 Monoclonal Antibody

Cat. Num. AAA190644

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Applications: ELISA, Immunoblot, Western Blot
• Synonyms: GRP78; Monoclonal Antibody; GRP78 Monoclonal Antibody; Anti-GRP78; anti-GRP78 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes human, mouse, and rat GRP78.
• Sequence Length: 653

• Applicable Applications for anti-GRP78 antibody: ELISA (EIA), Immunoblot (IB)
• Application Notes: Immunoblotting: Use at 1ug/ml. A band of ~78kDa is detected.
• Antigen: Full-length recombinant rat GRP78.
• Positive Control: HEK293 cell lysate.
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Testing Data

Related Product Information for anti-GRP78 antibody

GRP78 is a ubiquitously expressed, 78kDa glucose-regulated protein commonly referred to as an immunoglobulin chain binding protein (BiP). Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimers disease patients.

Product Categories/Family for anti-GRP78 antibody

Heat Shock and Stress Protein Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 386758
• NCBI GeneID: 3309
• Molecular Weight: 72,333 Da
• NCBI Official Full Name: GRP78, partial
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 5
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• Protein Family: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; focal adhesion; integral to endoplasmic reticulum membrane; melanosome; membrane; midbody; mitochondrion; myelin sheath; nucleus; plasma membrane; signalosome; smooth endoplasmic reticulum

Molecular Function: ATP binding; ATPase activity; calcium ion binding; chaperone binding; enzyme binding; glycoprotein binding; misfolded protein binding; protein binding; protein domain specific binding; ribosome binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: cellular response to glucose starvation; cerebellar Purkinje cell layer development; cerebellum structural organization; ER overload response; ER-associated protein catabolic process; negative regulation of apoptosis; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; positive regulation of protein ubiquitination; substantia nigra development; unfolded protein response; unfolded protein response, activation of signaling protein activity