Western Blot (WB)
(Western blot analysis of OLIG3 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). OLIG3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse OLIG3 Polyclonal Antibody | anti-OLIG3 antibody
OLIG3 Antibody (Center)
Gene Names
OLIG3; Bhlhb7; bHLHe20
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
OLIG3; Polyclonal Antibody; OLIG3 Antibody (Center); Oligodendrocyte transcription factor 3; Oligo3; Class B basic helix-loop-helix protein 7; bHLHb7; Class E basic helix-loop-helix protein 20; bHLHe20; BHLHB7; BHLHE20; anti-OLIG3 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OLIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the Central region of human OLIG3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
74-100
Sequence Length
272
Applicable Applications for anti-OLIG3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of OLIG3 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). OLIG3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of OLIG3 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). OLIG3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue with OLIG3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue with OLIG3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(OLIG3 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(OLIG3 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-OLIG3 antibody
OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Product Categories/Family for anti-OLIG3 antibody
References
Muller,T., et.al., Genes Dev. 19 (6), 733-743 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29358
NCBI Official Full Name
oligodendrocyte transcription factor 3
NCBI Official Synonym Full Names
oligodendrocyte transcription factor 3
NCBI Official Symbol
OLIG3
NCBI Official Synonym Symbols
Bhlhb7; bHLHe20
NCBI Protein Information
oligodendrocyte transcription factor 3
UniProt Protein Name
Oligodendrocyte transcription factor 3
Protein Family
UniProt Gene Name
OLIG3
UniProt Synonym Gene Names
BHLHB7; BHLHE20; Oligo3; bHLHb7; bHLHe20
UniProt Entry Name
OLIG3_HUMAN
Uniprot Description
OLIG3: May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Chromosomal Location of Human Ortholog: 6q23.3
Cellular Component: nucleus
Molecular Function: protein dimerization activity; DNA binding
Biological Process: transcription, DNA-dependent; spinal cord motor neuron cell fate specification; negative regulation of transcription from RNA polymerase II promoter
Research Articles on OLIG3
Similar Products
Product Notes
The OLIG3 olig3 (Catalog #AAA9202387) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 74-100. The OLIG3 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OLIG3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the OLIG3 olig3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OLIG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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