Western Blot (WB)
(Western blot analysis of GPC6 (arrow) using rabbit polyclonal GPC6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.)
Rabbit anti-Human GPC6 Polyclonal Antibody | anti-GPC6 antibody
GPC6, CT (GPC6, Glypican-6, Secreted glypican-6) (MaxLight 405)
Gene Names
GPC6; OMIMD1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
GPC6; Polyclonal Antibody; CT (GPC6; Glypican-6; Secreted glypican-6) (MaxLight 405); Secreted glypican-6; anti-GPC6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-GPC6 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
GPC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-536 amino acids from the C-terminal region of human GPC6.
Conjugate
MaxLight405
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of GPC6 (arrow) using rabbit polyclonal GPC6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.)
Western Blot (WB)
(Western blot analysis of GPC6 (arrow) using rabbit polyclonal GPC6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.)
Immunohistochemistry (IHC)
(GPC6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GPC6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GPC6 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GPC6 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GPC6 antibody
The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq].
Product Categories/Family for anti-GPC6 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lau, C.S., et al. Br J Ophthalmol 94(3):357-362(2010). Calboli, F.C., et al. PLoS ONE 5 (7), E11504 (2010): Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Campos-Xavier, A.B., et al. Am. J. Hum. Genet. 84(6):760-770(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,736 Da
NCBI Official Full Name
glypican-6
NCBI Official Synonym Full Names
glypican 6
NCBI Official Symbol
GPC6
NCBI Official Synonym Symbols
OMIMD1
NCBI Protein Information
glypican-6
UniProt Protein Name
Glypican-6
Protein Family
UniProt Gene Name
GPC6
UniProt Entry Name
GPC6_HUMAN
NCBI Description
The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Mutations in this gene are associated with omodysplasia 1. [provided by RefSeq, Nov 2016]
Research Articles on GPC6
Similar Products
Product Notes
The GPC6 gpc6 (Catalog #AAA6303485) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPC6, CT (GPC6, Glypican-6, Secreted glypican-6) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPC6 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GPC6 gpc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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