SDS-Page
(Figure. SDS-PAGE)
Fibrillin 1 Active Protein | FBN1 active protein
Active Fibrillin 1 (FBN1)
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Western Blot (WB)
(Figure. Western Blot)
Western Blot (WB)
(Figure. Western Blot)
Biological Activity Testing
(Figure. The binding activity of FBN1 with FBLN2.Fibrillin 1 (FBN1) is a 230-kb gene with 65 coding exons that encode a 2,871-amino-acid long proprotein called profibrillin which is proteolytically cleaved near its C-terminus by the enzyme furin convertase to give fibrillin-1, a member of the fibrillin family, and the 140-amino-acid long protein hormone asprosin. FBN1 is a large, extracellular matrix glycoprotein that serves as a structural component of 10-12nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Besides, Fibulin 2 (FBLN2) has been identified as an interactor of FBN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FBN1 and recombinant mouse FBLN2. Briefly, FBN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to FBLN2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-FBN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FBN1 and FBLN2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Biological Activity Testing
(Figure. The binding activity of FBN1 with FBLN2.Fibrillin 1 (FBN1) is a 230-kb gene with 65 coding exons that encode a 2,871-amino-acid long proprotein called profibrillin which is proteolytically cleaved near its C-terminus by the enzyme furin convertase to give fibrillin-1, a member of the fibrillin family, and the 140-amino-acid long protein hormone asprosin. FBN1 is a large, extracellular matrix glycoprotein that serves as a structural component of 10-12nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Besides, Fibulin 2 (FBLN2) has been identified as an interactor of FBN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FBN1 and recombinant mouse FBLN2. Briefly, FBN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to FBLN2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-FBN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FBN1 and FBLN2 was shown in Figure 1, and this effect was in a dose dependent manner.)
NCBI and Uniprot Product Information
Accurate Molecular Mass: 21kDa
Uniprot Description
FBN1: a large, extracellular matrix glycoprotein of the fibrillin family that serves as a structural component of 10-12 nm calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1- containing microfibrils provide long-term force bearing structural support in elastic and nonelastic connective tissue throughout the body. May regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively. Defects in this protein are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. Interacts with COL16A1.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; microfibril; basement membrane
Molecular Function: protein binding; extracellular matrix structural constituent; calcium ion binding
Biological Process: heart development; kidney development; metanephros development; skeletal development
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Product Notes
The FBN1 fbn1 (Catalog #AAA2122314) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Leu457~Asp634. AAA Biotech's Fibrillin 1 can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the FBN1 fbn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibrillin 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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