Rabbit anti-Human GPC6 Polyclonal Antibody | anti-GPC6 antibody

GPC6 Antibody (C-term)

Cat. Num. AAA9208676

• Gene Names: GPC6; OMIMD1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GPC6; Polyclonal Antibody; GPC6 Antibody (C-term); Glypican-6; Secreted glypican-6; anti-GPC6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GPC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-528 amino acids from the C-terminal region of human GPC6.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 496-528
• Sequence Length: 555

• Applicable Applications for anti-GPC6 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of GPC6 (arrow) using rabbit polyclonal GPC6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.)

Immunohistochemistry (IHC)

(GPC6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GPC6 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPC6 antibody

The glypicans comprise a family of
glycosylphosphatidylinositol-anchored heparan sulfate
proteoglycans, and they have been implicated in the control of cell
growth and cell division. The glypican encoded by this gene is a
putative cell surface coreceptor for growth factors, extracellular
matrix proteins, proteases and anti-proteases. [provided by
RefSeq].

Product Categories/Family for anti-GPC6 antibody

Signal Transduction

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lau, C.S., et al. Br J Ophthalmol 94(3):357-362(2010)
Calboli, F.C., et al. PLoS ONE 5 (7), E11504 (2010) :
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Campos-Xavier, A.B., et al. Am. J. Hum. Genet. 84(6):760-770(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 5031719
• NCBI GeneID: 10082
• NCBI Accession #: NP_005699.1
• NCBI GenBank Nucleotide #: NM_005708.3
• UniProt Accession #: Q9Y625; Q96SG5; Q96SG8; Q9H1P4; A8K279
• Molecular Weight: 62736
• NCBI Official Full Name: glypican-6
• NCBI Official Synonym Full Names: glypican 6
• NCBI Official Symbol: GPC6
• NCBI Official Synonym Symbols: OMIMD1
• NCBI Protein Information: glypican-6
• UniProt Protein Name: Glypican-6
• Protein Family: Glypican
• UniProt Gene Name: GPC6
• UniProt Entry Name: GPC6_HUMAN

NCBI Description

The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq, Jan 2009]

Uniprot Description

GPC6: Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling. Defects in GPC6 are a cause of omodysplasia type 1 (OMOD1). OMOD1 is a rare autosomal recessive skeletal dysplasia characterized by severe congenital micromelia with shortening and distal tapering of the humeri and femora to give a club-like appearance. Typical facial features include a prominent forehead, frontal bossing, short nose with a depressed broad bridge, short columella, anteverted nostrils, long philtrum, and small chin. Point mutations leading to protein truncation, as well as larger genomic rearrangements resulting in exon deletions, have been found in family segregating omodysplasia type 1. All mutations identified in individuals affected by omodysplasia could lead to the absence of a functional protein, the mutant RNAs being suspected to be nonsense-mediated mRNA decay (NMD) targets. Even if the mRNA escapes NMD and is translated, all mutations are expected to disrupt the three-dimensional protein structure and often to abolish multiple highly conserved cysteine residues. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; phototransduction, visible light; cell migration; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Omodysplasia 1