SDS-Page
Laminin Beta 4 Recombinant Protein | LAMb4 recombinant protein
Recombinant Laminin Beta 4 (LAMb4)
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Laminin Beta 4; Recombinant Laminin Beta 4 (LAMb4); LAMb4 recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 300ug/mL (varies by lot)
Sequence
NEEADGAQKLLVKAKAAE KAANILLNLD KTLNQLQQAQ ITQGRANSTI TQLTANITKIKKNVLQAENQ TREMKSELEL AKQRSGLEDG LSLLQTKLQR HQDHAVNAKVQAESAQHQAG SLEKEFVELK KQYAILQRKT STTGLTKETL GKVKQLKDAAEKLAGDTEAK IRRITDLERK IQDLNLSRQA KADQLRILED QVVAIKNEIVEQEKKYARC
Applicable Applications for LAMb4 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Asn1543~Cys1759
Tags
N-terminal His Tag
Subcellular Location
Secreted, Extracellular matrix
Traits
Freeze-dried powder
Predicted isoelectric point
9.4
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted Molecular Mass: 28.0KDa
Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
laminin, beta 4
NCBI Official Synonym Full Names
laminin, beta 4
NCBI Official Symbol
LAMB4
NCBI Protein Information
laminin subunit beta-4
UniProt Protein Name
Laminin subunit beta-4
Protein Family
UniProt Gene Name
LAMB4
UniProt Entry Name
LAMB4_HUMAN
Uniprot Description
LAMB4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7q31
Cellular Component: basement membrane
Biological Process: cell adhesion
Research Articles on LAMb4
Similar Products
Product Notes
The LAMb4 lamb4 (Catalog #AAA2031766) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Laminin Beta 4 (LAMb4) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin Beta 4 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the LAMb4 lamb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NEEADGAQKL LVKAKAAE KAANILLNLD KTLNQLQQAQ ITQGRANSTI TQLTANITKI KKNVLQAENQ TREMKSELEL AKQRSGLEDG LSLLQTKLQR HQDHAVNAKV QAESAQHQAG SLEKEFVELK KQYAILQRKT STTGLTKETL GKVKQLKDAA EKLAGDTEAK IRRITDLERK IQDLNLSRQA KADQLRILED QVVAIKNEIV EQEKKYARC. It is sometimes possible for the material contained within the vial of "Laminin Beta 4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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