anti-Rat Glutamate, Vesicular Transporters 1, CT Polyclonal Antibody
Anti - Glutamate, Vesicular Transporters 1, CT (VGLUT1, BNPI)
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Glutamate; Vesicular Transporters 1; CT; Polyclonal Antibody; Anti - Glutamate; CT (VGLUT1; BNPI); anti-Glutamate; CT antibody
Host
Host: Chicken
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
The rat peptide is 93% conserved in human VGLUT1. No significant sequence homology is observed with VGLUT2, EAT-4, or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-Glutamate, Vesicular Transporters 1, CT antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 19 AA synthetic peptide witin the C-terminus of rat VGLUT1 was synthesized, conjugated to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Glutamate, Vesicular Transporters 1, CT antibody
Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Most recently, vesicular type transporters for glutamate, termed VGLUTs (VGLUT1/BNPI, VGLUT2/DNPI, and EAT-4), have been cloned and characterized that are related to phosphate transporters. Although neurons exhibit Na-dependent Pi transport, the biological role of Pi uptake is not clear. Proximal tubules in the kidney reabsorb Pi in the glomeruli by the action of a group of phosphate transporters (Type 1-NaPi related, type 2-NaPi-2 related, and type 3-viral receptor-related. These receptors show weak (~20% identity) between various subtypes. A distinct type of brain specific Na+-dependent phosphate (Pi) transporter (BNPI), originally characterized as a plasma membrane transporter has been localized in a subset of glutamatergic neurons (amygdla,cereberal cortex, and hippocmaplus) and identified as VGLUT1 (rat and human 560 aa; ~60kD, ~30% homology with type-1 Pi-transporters & ~75-80% homology with VGLUT2). Interestingly, a sequence induced by subtoxic levels of NMDA in cerebelar granules also belongs to this family of transporters. BNPI/VGLUT1 is expression is restricted to the brain, where it is predominantly located in synaptic vesicles. VGLUT1 shows strong sequence homology (~48%) to EAT-4, a C. elegans protein that appears to have specific presynaptioc role in glutamtergic transmission.
Product Categories/Family for anti-Glutamate, Vesicular Transporters 1, CT antibody
References
• 1. Aihara Y et al (2000) J. Neurochem. 74, 2622-2625; Bellocchio EE et al (2000) Science 289, 957-960; Bellocchio EE et al (1998) J. Neurosci. 18, 8648; Ni B et al (1996) J. Neurochem. 66, 2227-2238; Takamori S et al (2000) Nature 407, 189-194
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Product Notes
The Glutamate, Vesicular Transporters 1, CT (Catalog #AAA610436) is an Antibody produced from Host: Chicken Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - Glutamate, Vesicular Transporters 1, CT (VGLUT1, BNPI) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate, Vesicular Transporters 1, CT can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2). ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Glutamate, Vesicular Transporters 1, CT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate, Vesicular Transporters 1, CT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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