Human TATA-box-binding protein-like protein 1 (TRF1) ELISA Kit | TRF1 elisa kit

Human TATA-box-binding protein-like protein 1 (TRF1) ELISA Kit

Cat. Num. AAA167407

• Gene Names: TBPL1; TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
• Reactivity: Human
• Synonyms: TATA-box-binding protein-like protein 1 (TRF1); Human TATA-box-binding protein-like protein 1 (TRF1) ELISA Kit; TRF1 elisa kit

• Reactivity: Human
• Sequence Length: 186

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 30ng/ml
• Sensitivity: 0.023ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRF1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human TATA-box-binding Protein-like Protein 1 (also known as Trf1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Trf1 antibody. Trf1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Trf1 Antibody is added and binds to Trf1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Trf1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Trf1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 358439409
• NCBI GeneID: 9519
• NCBI Accession #: NP_001240605.1
• NCBI GenBank Nucleotide #: NM_001253676.1
• UniProt Accession #: P62380; O95753; Q9BWD5; Q9Z2Z0; A8K8F5
• Molecular Weight: 20,887 Da
• NCBI Official Full Name: TATA box-binding protein-like protein 1
• NCBI Official Synonym Full Names: TATA-box binding protein like 1
• NCBI Official Symbol: TBPL1
• NCBI Official Synonym Symbols: TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
• NCBI Protein Information: TATA box-binding protein-like protein 1
• UniProt Protein Name: TATA box-binding protein-like protein 1
• Protein Family: Telomeric DNA-binding factor
• UniProt Gene Name: TBPL1
• UniProt Synonym Gene Names: TLF; TLP; TLP21; TRF2; TRP; TBP-like protein 1; STUD; TBP-related factor 2

NCBI Description

This gene encodes a member of the TATA box-binding protein family. TATA box-binding proteins play a critical role in transcription by RNA polymerase II as components of the transcription factor IID (TFIID) complex. The encoded protein does not bind to the TATA box and initiates transcription from TATA-less promoters. This gene plays a critical role in spermatogenesis, and single nucleotide polymorphisms in this gene may be associated with male infertility. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. [provided by RefSeq, Nov 2011]

Uniprot Description

Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.