Western Blot (WB)
(Western blot analysis of anti-GIT1 Antibody (Y510) in mouse cerebellum tissue lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.)
Rabbit GIT1 Polyclonal Antibody | anti-GIT1 antibody
GIT1 Antibody (Y510)
Western Blot (WB)
(Western blot analysis of anti-GIT1 Antibody (Y510) in mouse cerebellum tissue lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-GIT1 Antibody (Y510) in mouse cerebellum tissue lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GIT1 Antibody (Y510) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GIT1 Antibody (Y510) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Jones,N.P., Mol. Cell. Biol. 27 (16), 5790-5805 (2007)
Schmalzigaug,R., Cell. Signal. 19 (8), 1733-1744 (2007)
Stockton,R., Mol. Biol. Cell 18 (6), 2346-2355 (2007)
NCBI and Uniprot Product Information
Uniprot Description
GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).
Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, ARF
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: focal adhesion; membrane; cytoplasm; cytosol
Molecular Function: protein binding; zinc ion binding
Biological Process: axon guidance; ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
Research Articles on GIT1
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Product Notes
The GIT1 git1 (Catalog #AAA9213532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 487-517. The GIT1 Antibody (Y510) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GIT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the GIT1 git1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GIT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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