Rabbit anti-Human GIT1 Polyclonal Antibody | anti-GIT1 antibody

GIT1 Antibody

Cat. Num. AAA9408882

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: GIT1; Polyclonal Antibody; GIT1 Antibody; ARF GTPase-activating protein GIT1; G protein-coupled receptor kinase-interactor 1; similar to G protein-coupled receptor kinase-interactor 1; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein; anti-GIT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total GIT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 770

• Applicable Applications for anti-GIT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from C-terminal of human GIT1.
• Target Name: GIT1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using GIT1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GIT1 antibody.)

Related Product Information for anti-GIT1 antibody

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.Premont R.T., J. Biol. Chem. 275:22373-22380(2000).The MGC Project Team, Genome Res. 14:2121-2127(2004).Premont R.T., Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998).

Product Categories/Family for anti-GIT1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 146231998
• NCBI GeneID: 28964
• NCBI Accession #: NP_001078923.1
• NCBI GenBank Nucleotide #: NM_001085454.1
• UniProt Accession #: Q9Y2X7; Q86SS0; Q9BRJ4; B4DGU9; B4DSV3
• Molecular Weight: 85,446 Da
• NCBI Official Full Name: ARF GTPase-activating protein GIT1 isoform 1
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase interacting ArfGAP 1
• NCBI Official Symbol: GIT1
• NCBI Protein Information: ARF GTPase-activating protein GIT1
• UniProt Protein Name: ARF GTPase-activating protein GIT1
• Protein Family: ARF GTPase-activating protein
• UniProt Gene Name: GIT1
• UniProt Synonym Gene Names: ARF GAP GIT1; CAT-1; CAT1
• UniProt Entry Name: GIT1_HUMAN

Uniprot Description

GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).

Protein type: GAPs, ARF; GAPs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: focal adhesion; membrane; cytoplasm; cytosol

Molecular Function: protein binding; zinc ion binding

Biological Process: axon guidance; ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity