Mouse Interleukin 1 Alpha (IL1a) ELISA Kit | IL1a elisa kit

Mouse Interleukin 1 Alpha (IL1a) Mini Samples ELISA Kit

Cat. Num. AAA2708224

• Gene Names: Il1a; Il-1a
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Interleukin 1 Alpha (IL1a); Mouse Interleukin 1 Alpha (IL1a) Mini Samples ELISA Kit; Interleukin 1 Alpha; IL1-A; IL-1alpha; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1; IL1a elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 1 Alpha (IL1a).; No significant cross-reactivity or interference between Mini Samples Interleukin 1 Alpha (IL1a) and analogues was observed.
• Sequence Length: 270

• Applicable Applications for IL1a elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 3.1pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 1 Alpha (IL1a) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 1 Alpha (IL1a) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 47059075
• NCBI GeneID: 16175
• NCBI Accession #: NP_034684.2
• NCBI GenBank Nucleotide #: NP_034684.2
• UniProt Accession #: P01582
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: Il1a
• NCBI Official Synonym Symbols: Il-1a
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il1a
• UniProt Synonym Gene Names: IL-1 alpha
• UniProt Entry Name: IL1A_MOUSE

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Cytokine; Motility/polarity/chemotaxis

Cellular Component: cell surface; cytoplasm; cytosol; extracellular space; plasma membrane

Molecular Function: copper ion binding; cytokine activity; interleukin-1 receptor binding

Biological Process: connective tissue replacement during inflammatory response; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; inflammatory response; keratinization; negative regulation of cell proliferation; positive regulation of angiogenesis; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of mitosis; positive regulation of prostaglandin secretion; positive regulation of protein secretion; positive regulation of stress-activated MAPK cascade; positive regulation of transcription from RNA polymerase II promoter; regulation of actin cytoskeleton organization and biogenesis; regulation of sensory perception of pain; response to copper ion