Western Blot (WB)
(Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).)
Mouse EGFP/EYFP Monoclonal Antibody | anti-EGFP/EYFP antibody
EGFP/EYFP Monoclonal Antibody
Reactivity
Bacillus cereus
Applications
Western Blot
Purity
Affinity purified
Synonyms
EGFP/EYFP; Monoclonal Antibody; EGFP/EYFP Monoclonal Antibody; GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein; anti-EGFP/EYFP antibody
Host
Mouse
Reactivity
Bacillus cereus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F11-B12-E7-F12
Specificity
Transfected
Purity/Purification
Affinity purified
Form/Format
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Sequence Length
359
Applicable Applications for anti-EGFP/EYFP antibody
Western Blot (WB)
Application Notes
Western blotting: 1:1000
Immunogen Type
Recombinant Protein
Immunogen Description
Purified recombinant EYFP.tag full length expressed in E Coli.
Target Name
EGFP/EYFP
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).)
Western Blot (WB)
(Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).)
Western Blot (WB)
(Western blot detection of EYFP expression in Rosetta E Coli cells induced by 0(A)or 0.1mM (B)IPTG using EGFP antibody(1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.)
Western Blot (WB)
(Western blot detection of EYFP expression in Rosetta E Coli cells induced by 0(A)or 0.1mM (B)IPTG using EGFP antibody(1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.)
Western Blot (WB)
(Western blot detection of EGFP expression in Hela cells non-transfected (A) or transfected (B) with pEGFP C1 using EGFP antibody(1:5000 diluted).Predicted band size: 34KDa Observed band size: 34KDa.)
Western Blot (WB)
(Western blot detection of EGFP expression in Hela cells non-transfected (A) or transfected (B) with pEGFP C1 using EGFP antibody(1:5000 diluted).Predicted band size: 34KDa Observed band size: 34KDa.)
Related Product Information for anti-EGFP/EYFP antibody
The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.
Product Categories/Family for anti-EGFP/EYFP antibody
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
40,870 Da
UniProt Protein Name
Enhanced green fluorescent protein
UniProt Gene Name
eGFP
UniProt Entry Name
C8CHS1_BACCE
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Product Notes
The EGFP/EYFP egfp (Catalog #AAA9400338) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EGFP/EYFP Monoclonal Antibody reacts with Bacillus cereus and may cross-react with other species as described in the data sheet. AAA Biotech's EGFP/EYFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:1000. Researchers should empirically determine the suitability of the EGFP/EYFP egfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFP/EYFP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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