Western Blot
(Western Blot analysis using GFP antibodyLane 1 - Marker; Lane - GP fusion protein; Lane 3 - Control)
Rabbit GFP Polyclonal Antibody | anti-GFP antibody
GFP antibody
Reactivity
This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP
Applications
Immunoprecipitation, Western Blot
Synonyms
GFP; Polyclonal Antibody; GFP antibody; Polyclonal GFP; Anti-GFP; Green Fluorescent Protein; anti-GFP antibody
Host
Rabbit
Reactivity
This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP
Clonality
Polyclonal
Form/Format
Supplied as a lyophilized Potein A purified rabbit IgG with 0.1% NaN3. Reconstitue in distilled water.
Sequence Length
238
Applicable Applications for anti-GFP antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 1:200-1:500
WB: 1:5,000
WB: 1:5,000
Biological Significance
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Cross-Reactivity
This antibody reacts with wild -type GFP and it’s variants, such as EGFP, EBFP
Immunogen
GFP antibody was raised in rabbit using E Coli-expressed full length green fluorescent protein as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot
(Western Blot analysis using GFP antibodyLane 1 - Marker; Lane - GP fusion protein; Lane 3 - Control)
Western Blot
(Western Blot analysis using GFP antibodyLane 1 - Marker; Lane - GP fusion protein; Lane 3 - Control)
Related Product Information for anti-GFP antibody
Rabbit polyclonal GFP antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
26,886 Da
NCBI Official Full Name
Green fluorescent protein
UniProt Protein Name
Green fluorescent protein
Protein Family
UniProt Gene Name
GFP
UniProt Entry Name
GFP_AEQVI
Uniprot Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
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Product Notes
The GFP gfp (Catalog #AAA534482) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GFP antibody reacts with This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 1:200-1:500 WB: 1:5,000. Researchers should empirically determine the suitability of the GFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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