GFP PicoKine ELISA Kit | GFP elisa kit

GFP PicoKine ELISA Kit

Cat. Num. AAA177564

• Synonyms: GFP PicoKine; GFP PicoKine ELISA Kit; Green fluorescent protein; GFP; yfp; GFP elisa kit

• Specificity: Natural and recombinant All Animals GFP
• Sequence Length: 238

• Samples: Cell lysates and tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(GFP PicoKine ELISA Kit standard curve )

Related Product Information for GFP elisa kit

Principle of the Assay: All Animals GFP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for GFP has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: S2-K238) and test samples are added to the wells, a biotinylated detection specific for GFP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the All Animals GFP amount of sample captured in plate.

Background: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.

NCBI and Uniprot Product Information

• NCBI GI #: 1169893
• UniProt Accession #: P42212; Q17104; Q27903; Q93125
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: Green fluorescent protein
• UniProt Protein Name: Green fluorescent protein
• Protein Family: Green fluorescent protein
• UniProt Gene Name: GFP
• UniProt Entry Name: GFP_AEQVI

Uniprot Description

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.