Western Blot (WB)
(Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. GFP antibody (HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP.)
Goat GFP Polyclonal Antibody | anti-GFP antibody
GFP antibody (HRP)
Reactivity
Jellyfish GFP
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
GFP antibody (HRP) was purified by affinity chromatography.
Synonyms
GFP; Polyclonal Antibody; GFP antibody (HRP); Polyclonal GFP; Anti-GFP; Green Fluorescent Protein; GFP conjugate; anti-GFP antibody
Host
Goat
Reactivity
Jellyfish GFP
Clonality
Polyclonal
Specificity
Jellyfish GFP
Purity/Purification
GFP antibody (HRP) was purified by affinity chromatography.
Form/Format
Lyophilized from 0.02M K2PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
Sequence Length
238
Applicable Applications for anti-GFP antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:10,000-1:50,000
IHC: 1:500-1:2,000
WB: 1:2,000 - 1:5,000
IHC: 1:500-1:2,000
WB: 1:2,000 - 1:5,000
Tag/Conjugate
HRP
Immunogen
GFP antibody (HRP) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. GFP antibody (HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP.)
Western Blot (WB)
(Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. GFP antibody (HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP.)
Related Product Information for anti-GFP antibody
Goat polyclonal GFP antibody (HRP) conjugated
Product Categories/Family for anti-GFP antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
26,886 Da
NCBI Official Full Name
Green fluorescent protein
UniProt Protein Name
Green fluorescent protein
Protein Family
UniProt Gene Name
GFP
UniProt Entry Name
GFP_AEQVI
Uniprot Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
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Product Notes
The GFP gfp (Catalog #AAA534313) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The GFP antibody (HRP) reacts with Jellyfish GFP and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:10,000-1:50,000 IHC: 1:500-1:2,000 WB: 1:2,000 - 1:5,000. Researchers should empirically determine the suitability of the GFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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