Human Synaptophysin (SYP) ELISA Kit | SYP elisa kit

Human Synaptophysin (SYP) ELISA Kit

Cat. Num. AAA2086973

• Gene Names: SYP; MRX96; MRXSYP
• Reactivity: Human
• Synonyms: Synaptophysin (SYP); Human Synaptophysin (SYP) ELISA Kit; Major synaptic vesicle protein p38; SYP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Synaptophysin (SYP).; No significant cross-reactivity or interference between Synaptophysin (SYP) and analogues was observed.
• Sequence Length: 313

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 246.9-20,000pg/mL
• Sensitivity: < 91.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptophysin (SYP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptophysin (SYP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SYP elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Synaptophysin (SYP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Synaptophysin (SYP) and unlabeled Synaptophysin (SYP) (Standards or samples) with the pre-coated antibody specific to Synaptophysin (SYP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Synaptophysin (SYP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Synaptophysin (SYP) in the sample.

Product Categories/Family for SYP elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 27764867
• NCBI GeneID: 6855
• NCBI Accession #: NP_003170.1
• NCBI GenBank Nucleotide #: NM_003179.2
• UniProt Accession #: P08247; Q6P2F7; B2R7L6; B7Z359
• Molecular Weight: 20,757 Da
• NCBI Official Full Name: synaptophysin
• NCBI Official Synonym Full Names: synaptophysin
• NCBI Official Symbol: SYP
• NCBI Official Synonym Symbols: MRX96; MRXSYP
• NCBI Protein Information: synaptophysin
• UniProt Protein Name: Synaptophysin
• Protein Family: Synaptophysin
• UniProt Gene Name: SYP

NCBI Description

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Vesicle

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: integral to synaptic vesicle membrane; neuron projection; synaptic vesicle

Molecular Function: cholesterol binding; protein self-association

Biological Process: endocytosis; regulation of long-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity; regulation of short-term neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96