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Western Blot (WB) (Western blot under reducing conditions on 293 cell lysate using MBS617634 at a dilution of 1:100.)

Rabbit anti-Human, Rat GABARAP L2 Polyclonal Antibody

GABARAP L2 (Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, MAP1 light chain 3-related protein, ATG8, GATE16, GABARAPL2,

Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Serum
Serum
Synonyms
GABARAP L2; Polyclonal Antibody; GABARAP L2 (Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 3-related protein; ATG8; GATE16; GABARAPL2; ; Anti -GABARAP L2 (Gamma-aminobutyric acid receptor-associated protein-like 2; anti-GABARAP L2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
IHC, WB and ELISA confirmed the specificity for GABARAP L2. Species Crossreactivity: Human, rat.
Purity/Purification
Serum
Serum
Form/Format
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Applicable Applications for anti-GABARAP L2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Immunogen
A synthetic peptide corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2).
Cellular Localization
Golgi apparatus.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot under reducing conditions on 293 cell lysate using MBS617634 at a dilution of 1:100.)

Western Blot (WB) (Western blot under reducing conditions on 293 cell lysate using MBS617634 at a dilution of 1:100.)

Immunofluorescence (IF)

(A. Confocal microscopy on immunofluorescently detected MPO (in green) in cytospin-isolated human white blood cells counter-stained with Hoechst Dye. Here, the merged picture is presented.)

Immunofluorescence (IF) (A. Confocal microscopy on immunofluorescently detected MPO (in green) in cytospin-isolated human white blood cells counter-stained with Hoechst Dye. Here, the merged picture is presented.)

Immunofluorescence (IF)

(B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using MBS617634at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were stained for GABARAP L2 (GABARAPL2) appearing in red counter-stained with Hoechst DyeHere. The merged picture is presented.)

Immunofluorescence (IF) (B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using MBS617634at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were stained for GABARAP L2 (GABARAPL2) appearing in red counter-stained with Hoechst DyeHere. The merged picture is presented.)

Immunofluorescence (IF)

(C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using MBS617634 at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 (GABARAPL2) appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour).)

Immunofluorescence (IF) (C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using MBS617634 at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 (GABARAPL2) appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour).)

Western Blot (WB)

(Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using MBS617634 at a dilution of 1:100.)

Western Blot (WB) (Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using MBS617634 at a dilution of 1:100.)
Product Categories/Family for anti-GABARAP L2 antibody

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Product Notes

The GABARAP L2 (Catalog #AAA617634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GABARAP L2 (Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, MAP1 light chain 3-related protein, ATG8, GATE16, GABARAPL2, reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GABARAP L2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Researchers should empirically determine the suitability of the GABARAP L2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABARAP L2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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