Immunofluorescence (IF)
(Confocal microscopy on immunofluorescently detected APG10 in cytospin-isolated human white blood cells using MBS610643at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.)
Rabbit anti-Human Apg10 Polyclonal Antibody | anti-ATG10 antibody
Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)
Serum
Serum
Dilution: Immuhistochemistry (IF): 1:100-1:1000
Immunofluorescence (IF)
(Confocal microscopy on immunofluorescently detected APG10 in cytospin-isolated human white blood cells using MBS610643at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.)
Immunofluorescence (IF)
(Confocal microscopy on immunofluorescently detected APG10 in cytospin-isolated human white blood cells using MBS610643at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.)
NCBI and Uniprot Product Information
NCBI Description
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM]
Uniprot Description
ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Autophagy; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: cytoplasm; intracellular
Molecular Function: APG12 conjugating enzyme activity; ligase activity
Biological Process: protein transport; protein modification by small protein conjugation; protein amino acid lipidation; positive regulation of protein modification process; autophagy
Research Articles on ATG10
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Product Notes
The ATG10 atg10 (Catalog #AAA610643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apg10 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Dilution: Immuhistochemistry (IF): 1:100-1:1000. Researchers should empirically determine the suitability of the ATG10 atg10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apg10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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