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Western Blot (WB) (Fig. Western Blot analysis of extracts from K562 cells, using FTO Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)

Rabbit FTO Polyclonal Antibody | anti-FTO antibody

FTO Polyclonal Antibody

Gene Names
FTO; GDFD; ALKBH9; BMIQ14
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
FTO; Polyclonal Antibody; FTO Polyclonal Antibody; Rabbit Anti-FTO Polyclonal Antibody; KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; anti-FTO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
FTO Polyclonal Antibody detects endogenous levels of FTO protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
505
Applicable Applications for anti-FTO antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:20000
Immunogen
Synthesized peptide derived from the N-terminal region of human FTO
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig. Western Blot analysis of extracts from K562 cells, using FTO Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)

Western Blot (WB) (Fig. Western Blot analysis of extracts from K562 cells, using FTO Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)
Related Product Information for anti-FTO antibody
FTO is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase FTO isoform 3
NCBI Official Synonym Full Names
FTO alpha-ketoglutarate dependent dioxygenase
NCBI Official Symbol
FTO
NCBI Official Synonym Symbols
GDFD; ALKBH9; BMIQ14
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Gene Name
FTO
UniProt Synonym Gene Names
KIAA1752
UniProt Entry Name
FTO_HUMAN

NCBI Description

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]

Research Articles on FTO

Similar Products

Product Notes

The FTO fto (Catalog #AAA9703428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FTO Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FTO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:20000. Researchers should empirically determine the suitability of the FTO fto for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FTO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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