Western Blot (WB)
(Western blot (WB) analysis of FTO polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysate(40ug)LaneB: Hela whole cell lysate(40ug)LaneC: A549 whole cell lysate(40ug)LaneD: The rat lung lysate of Mouse(40ug)LaneE: The rat kindey lysate of Mouse(40ug))
Rabbit GPR224 Polyclonal Antibody | anti-GPR224 antibody
GPR224 polyclonal antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
GPR224; Polyclonal Antibody; GPR224 polyclonal antibody; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; KIAA1752; anti-GPR224 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
FTO polyclonal antibody detects endogenous levels of FTO protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GPR224 antibody
Western Blot (WB)
Application Notes
WB: 1:500~1:1000
Immunogen
Synthetic peptide, corresponding to Human FTO
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of FTO polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysate(40ug)LaneB: Hela whole cell lysate(40ug)LaneC: A549 whole cell lysate(40ug)LaneD: The rat lung lysate of Mouse(40ug)LaneE: The rat kindey lysate of Mouse(40ug))
Western Blot (WB)
(Western blot (WB) analysis of FTO polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysate(40ug)LaneB: Hela whole cell lysate(40ug)LaneC: A549 whole cell lysate(40ug)LaneD: The rat lung lysate of Mouse(40ug)LaneE: The rat kindey lysate of Mouse(40ug))
Related Product Information for anti-GPR224 antibody
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
505
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase FTO
NCBI Official Synonym Full Names
fat mass and obesity associated
NCBI Official Symbol
FTO
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; fat mass and obesity-associated protein
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Gene Name
FTO
UniProt Synonym Gene Names
KIAA1752
UniProt Entry Name
FTO_HUMAN
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Product Notes
The GPR224 fto (Catalog #AAA3015005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR224 polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR224 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500~1:1000. Researchers should empirically determine the suitability of the GPR224 fto for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR224, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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