Western Blot (WB)
(Western blot analysis of FOXO1 was performed by loading 25 ug of HeLa (lane 1), 293T (lane 2) and rat heart lysates (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4 degree C overnight. The membrane was probed with FOXO1 polyclonal antibody at a dilution of 1/500 overnight at 4 degree C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Detection was performed using chemiluminescence. Images were taken at an exposure time of 2 min (lanes 1 and 2) and 1 min (lane 3). Results show a band at ~66 kDa)
Rabbit anti-Human, Rat FOXO1 Polyclonal Antibody | anti-FOXO1 antibody
Rabbit anti FOXO1
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of FOXO1 was performed by loading 25 ug of HeLa (lane 1), 293T (lane 2) and rat heart lysates (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4 degree C overnight. The membrane was probed with FOXO1 polyclonal antibody at a dilution of 1/500 overnight at 4 degree C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Detection was performed using chemiluminescence. Images were taken at an exposure time of 2 min (lanes 1 and 2) and 1 min (lane 3). Results show a band at ~66 kDa)
Western Blot (WB)
(Western blot analysis of FOXO1 was performed by loading 25 ug of HeLa (lane 1), 293T (lane 2) and rat heart lysates (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4 degree C overnight. The membrane was probed with FOXO1 polyclonal antibody at a dilution of 1/500 overnight at 4 degree C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Detection was performed using chemiluminescence. Images were taken at an exposure time of 2 min (lanes 1 and 2) and 1 min (lane 3). Results show a band at ~66 kDa)
FOXO1 like other members of the forkhead protein family acts as a transcription factor by binding to DNA with its forkhead box protein domain. The subcellular activation and thereby the transcriptional activity of FOXO1 is regulated via AKT mediated phosphorylation while its levels are regulated via polyubiquitination.
FOXO1 has been shown to play a role in various signaling pathways ranging from apoptosis, proliferation, oxidative stress, DNA damage to insulin signaling (Huang and Tindall 2007). The protein has also been proposed to act as a tumor suppressor (Fu and Tindall 2008, Xie et al..2012).
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling ().
Research Articles on FOXO1
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Product Notes
The FOXO1 foxo1 (Catalog #AAA225047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti FOXO1 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FOXO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:100-1:1000. Researchers should empirically determine the suitability of the FOXO1 foxo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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