Mouse anti-Human Erythropoietin Receptor Monoclonal Antibody | anti-EPOR antibody

Erythropoietin Receptor, Phosphorylated (Y426) (EPOr, EPO-R)

Cat. Num. AAA648404

• Gene Names: EPOR; EPO-R
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: Erythropoietin Receptor; Monoclonal Antibody; Phosphorylated (Y426) (EPOr; EPO-R); Anti -Erythropoietin Receptor; anti-EPOR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 12K93
• Specificity: Recognizes human Erythropoietin R.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-EPOR antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Synthetic phosphopeptide corresponding to human Erythropoietin R at Y426 site.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human Phospho-Erythropoietin R (Y426) by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line untreated (­) ortreated (+) with 300ng/ml Recombinant Human Erythropoietin (Tissue Culture Grade) for 10 minutes. PVDF membrane was probed with 1ug/ml of Mouse Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 64-70kD. For additional reference, the membrane was stripped and reprobed with 1ug/ml of Mouse Human Erythropoietin R Monoclonal Antibody.)

Related Product Information for anti-EPOR antibody

Erythropoietin a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). A member of the hematopoietic growth factor receptor superfamily which includes IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, GM-CSF, G-CSF, Thrombopoietin, LIF, CNTF, Growth Hormone, and Prolactin, Epo R is expressed not only by erythroid cells but also by embryonic stem cells, endothelial cells, and neural cells. Mouse Epo R cDNA encode a type I membrane protein with 507aa residues. Mouse Epo R has a 24aa hydrophobic signal peptide, a 225aa extracellular domain, a 22aa transmembrane domain, and a 236aa intracellular domain. At the protein sequence level, the human Epo R is ~82% identical to the mouse protein. Mouse and human Epo R both contain 11 cysteine residues and an N-linked glycosylation site. Mouse Epo R, however, contains two disulfide bridges not found with human Epo R. In common with other hematopoietic growth factor receptor superfamily members, mouse Epo R has 4 positionally conserved cysteines in its extracellular domain, a tryptophan-serine-X- tryptophan-serine (WSXWS) motif or its homolog located near the transmembrane region, and lacks kinase motifs in its intracellular domain. Based on its aa composition the molecular weight of Epo R would be 55kD but after post translational modification including glycosylation and tyrosine and serine-threonine phosphorylation the molecular weight can be as high as 78kD. As a result of alternative splicing of the Epo R mRNA, cDNA clones encoding a truncated form of the Epo R as well as a soluble form of Epo R have been found. The presence of a soluble form of the Epo R has also been detected in human serum. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

Product Categories/Family for anti-EPOR antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4503591
• NCBI GeneID: 2057
• NCBI Accession #: NP_000112.1
• NCBI GenBank Nucleotide #: NM_000121.3
• UniProt Accession #: P19235; Q15443; Q2M205; B2RCG4
• Molecular Weight: 55,065 Da
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: EPOR
• NCBI Official Synonym Symbols: EPO-R
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: EPOR
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_HUMAN

NCBI Description

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Function: Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

Subunit structure: Forms homodimers on EPO stimulation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins including LYN

By similarity, the adapter protein APS, PTPN6

By similarity, PTPN11, JAK2, PI3 kinases, STAT5A/B, SOCS3, CRKL

By similarity. Interacts with INPP5D/SHIP1

By similarity. The N-terminal SH2 domain of PTPN6 binds Tyr-454 and inhibits signaling through dephosphorylation of JAK2

By similarity. APS binding also inhibits the JAK-STAT signaling. Binding to PTPN11, preferentially through the N-terminal SH2 domain, promotes mitogenesis and phosphorylation of PTPN11

By similarity. Binding of JAK2 (through its N-terminal) promotes cell-surface expression

By similarity. Interaction with the ubiquitin ligase NOSIP mediates EPO-induced cell proliferation. Interacts with ATXN2L. Ref.13 Ref.16 Ref.17 Ref.18 Ref.19

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.15. Isoform EPOR-S: Secreted. Note: Secreted and located to the cell surface. Ref.15

Tissue specificity: Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation.Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Post-translational modification: On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. Ref.18Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation

By similarity. Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

Involvement in disease: Familial erythrocytosis 1 (ECYT1) [MIM:133100]: An autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.25 Ref.26 Ref.27

Sequence similarities: Belongs to the type I cytokine receptor family. Type 1 subfamily.Contains 1 fibronectin type-III domain.