Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit | FOXO1 elisa kit

Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit

Cat. Num. AAA2020165

• Gene Names: FOXO1; FKH1; FKHR; FOXO1A
• Reactivity: Rat
• Synonyms: Forkhead Box Protein O1 (FOXO1); Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit; FOX-O1; FKH1; FKHR; FOXO1A; Forkhead Homolog In Rhabdomyosarcoma; FOXO1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein O1 (FOXO1).; No significant cross-reactivity or interference between Forkhead Box Protein O1 (FOXO1) and analogues was observed.
• Sequence Length: 655

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein O1 (FOXO1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein O1 (FOXO1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXO1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein O1 (FOXO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein O1 (FOXO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein O1 (FOXO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein O1 (FOXO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FOXO1 elisa kit

Signal transduction; Tumor immunity; Immune molecule; Developmental science; Autoimmunity

NCBI and Uniprot Product Information

• NCBI GI #: 9257222
• NCBI GeneID: 2308
• NCBI Accession #: NP_002006.2
• NCBI GenBank Nucleotide #: NM_002015.3
• UniProt Accession #: Q12778; O43523; Q5VYC7; Q6NSK6
• Molecular Weight: 69,662 Da
• NCBI Official Full Name: forkhead box protein O1
• NCBI Official Synonym Full Names: forkhead box O1
• NCBI Official Symbol: FOXO1
• NCBI Official Synonym Symbols: FKH1; FKHR; FOXO1A
• NCBI Protein Information: forkhead box protein O1
• UniProt Protein Name: Forkhead box protein O1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO1
• UniProt Synonym Gene Names: FKHR; FOXO1A
• UniProt Entry Name: FOXO1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: DNA-binding; Nuclear receptor co-regulator; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of fat cell differentiation; cellular response to starvation; negative regulation of stress-activated MAPK cascade; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; positive regulation of protein catabolic process; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; autophagy; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2