Rabbit anti-Human FOXN3 Polyclonal Antibody | anti-FOXN3 antibody

FOXN3, CT (FOXN3, C14orf116, CHES1, Forkhead box protein N3, Checkpoint suppressor 1) (Biotin)

Cat. Num. AAA6299907

• Gene Names: FOXN3; CHES1; PRO1635; C14orf116
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FOXN3; Polyclonal Antibody; CT (FOXN3; C14orf116; CHES1; Forkhead box protein N3; Checkpoint suppressor 1) (Biotin); Checkpoint suppressor 1; anti-FOXN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FOXN3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FOXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from the C-terminal region of human FOXN3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).FOXN3 immunoreactivity is localized to Nucleus significantly. )

Western Blot (WB)

(FOXN3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXN3 antibody detected the FOXN3 protein (arrow).)

Related Product Information for anti-FOXN3 antibody

This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq].

Product Categories/Family for anti-FOXN3 antibody

Antibodies; Transcription Factors

References

Samaan, G., et al. Biochem. Biophys. Res. Commun. 400(1):60-65(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Markowski, J., et al. Eur Arch Otorhinolaryngol 266(10):1501-1507(2009). Schlade-Bartusiak, K., et al. Am. J. Med. Genet. A 146A (1), 117-123 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 146231981
• NCBI GeneID: 1112
• NCBI Accession #: NP_005188.2
• NCBI GenBank Nucleotide #: NM_005197
• UniProt Accession #: O00409; Q96II7; Q9UIE7
• Molecular Weight: 51,534 Da
• NCBI Official Full Name: Homo sapiens forkhead box N3 (FOXN3), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: forkhead box N3
• NCBI Official Symbol: FOXN3
• NCBI Official Synonym Symbols: CHES1; PRO1635; C14orf116
• NCBI Protein Information: forkhead box protein N3; checkpoint suppressor 1
• UniProt Protein Name: Forkhead box protein N3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXN3
• UniProt Synonym Gene Names: C14orf116; CHES1
• UniProt Entry Name: FOXN3_HUMAN

NCBI Description

This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008]