Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FOXN3 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human FOXN3 Polyclonal Antibody | anti-FOXN3 antibody
FOXN3 Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FOXN3 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FOXN3 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(FOXN3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXN3 antibody detected the FOXN3 protein (arrow).)
Western Blot (WB)
(FOXN3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FOXN3 antibody detected the FOXN3 protein (arrow).)
transcription factor family. Checkpoints are eukaryotic DNA
damage-inducible cell cycle arrests at G1 and G2. Checkpoint
suppressor 1 suppresses multiple yeast checkpoint mutations
including mec1, rad9, rad53 and dun1 by activating a
MEC1-independent checkpoint pathway. Alternative splicing is
observed at the locus, resulting in distinct isoforms. [provided by
RefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Markowski, J., et al. Eur Arch Otorhinolaryngol 266(10):1501-1507(2009)
Schlade-Bartusiak, K., et al. Am. J. Med. Genet. A 146A (1), 117-123 (2008) :
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXN3: Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 14q31.3
Cellular Component: nucleus
Molecular Function: protein C-terminus binding; protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of transcription, DNA-dependent
Research Articles on FOXN3
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Product Notes
The FOXN3 foxn3 (Catalog #AAA9206226) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 380-408. The FOXN3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXN3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the FOXN3 foxn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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