Mouse anti-Human TTL Monoclonal Antibody | anti-TTL antibody

TTL Mouse Monoclonal

Cat. Num. AAA7605329

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Protein A+G purification
• Synonyms: TTL; Monoclonal Antibody; TTL Mouse Monoclonal; anti-TTL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: >=95% as determined by SDS-PAGE; Protein A+G purification
• Form/Format: Liquid
• Sequence Length: 377

• Applicable Applications for anti-TTL antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Tubulin tyrosine ligase
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using MBS7605329 (TTL antibody) at dilution of 1:50)

Western Blot (WB)

(SH-SY5Y cells were subjected to SDS PAGE followed by western blot with MBS7605329 (TTL antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 24308516
• NCBI GeneID: 150465
• NCBI Accession #: NP_714923.1
• NCBI GenBank Nucleotide #: NM_153712.4
• Molecular Weight: 43,212 Da
• NCBI Official Full Name: tubulin--tyrosine ligase
• NCBI Official Synonym Full Names: tubulin tyrosine ligase
• NCBI Official Symbol: TTL
• NCBI Protein Information: tubulin--tyrosine ligase
• UniProt Protein Name: Tubulin--tyrosine ligase
• Protein Family: TITAN-like protein
• UniProt Gene Name: TTL
• UniProt Synonym Gene Names: TTL
• UniProt Entry Name: TTL_HUMAN

NCBI Description

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008]

Uniprot Description

TTL: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin. Belongs to the tubulin--tyrosine ligase family.

Protein type: Ligase; EC 6.3.2.25; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2q13

Molecular Function: ATP binding; tubulin-tyrosine ligase activity

Biological Process: microtubule cytoskeleton organization and biogenesis; protein modification process; regulation of axon extension