Testing Data
(Paraffin embedded HEK293 cells transfected with SULF2 stained with Mouse anti Human Sulfatase 2 C-terminal (MBS224444))
Mouse SULFATASE 2 Monoclonal Antibody | anti-Sulf2 antibody
MOUSE ANTI HUMAN SULFATASE 2 (C-TERMINAL)
Gene Names
Sulf2; MSulf-2; AU020235; mKIAA1247; 2010004N24Rik
Reactivity
MouseN.B Antibody reactivity and working conditions may vary between species.
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
SULFATASE 2; Monoclonal Antibody; MOUSE ANTI HUMAN SULFATASE 2 (C-TERMINAL); SULF-2; KIAA1247; anti-Sulf2 antibody
Host
Mouse
Reactivity
Mouse
N.B Antibody reactivity and working conditions may vary between species.
N.B Antibody reactivity and working conditions may vary between species.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B4
Specificity
SULFATASE 2(C-Terminal)
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
875
Applicable Applications for anti-Sulf2 antibody
ELISA (EIA), Immunohistology Paraffin , Western Blot (WB)
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Preservative Stabilisers
0.09% Sodium Azide.
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Immunogen
Recombinant human Sulf-2.<
Histology Positive Control Tissue
Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
Target Species
Human
Buffer Solution
Phosphate buffered saline.
Preparation and Storage
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Testing Data
(Paraffin embedded HEK293 cells transfected with SULF2 stained with Mouse anti Human Sulfatase 2 C-terminal (MBS224444))
Testing Data
(Paraffin embedded HEK293 cells transfected with SULF2 stained with Mouse anti Human Sulfatase 2 C-terminal (MBS224444))
Related Product Information for anti-Sulf2 antibody
Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Studies have linked Sulf-2 with several cancers, including breast, hepatocellular, and pancreatic cancer, and recently with non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
extracellular sulfatase Sulf-2 isoform 1
NCBI Official Synonym Full Names
sulfatase 2
NCBI Official Symbol
Sulf2
NCBI Official Synonym Symbols
MSulf-2; AU020235; mKIAA1247; 2010004N24Rik
NCBI Protein Information
extracellular sulfatase Sulf-2
UniProt Protein Name
Extracellular sulfatase Sulf-2
Protein Family
UniProt Gene Name
Sulf2
UniProt Synonym Gene Names
mSulf-2
UniProt Entry Name
SULF2_MOUSE
Uniprot Description
SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.6.-; Secreted; Hydrolase; Secreted, signal peptide
Cellular Component: Golgi apparatus; extracellular space; cell surface; endoplasmic reticulum; plasma membrane
Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; hydrolase activity; sulfuric ester hydrolase activity; metal ion binding; calcium ion binding; catalytic activity; alkyl sulfatase activity
Biological Process: sulfur metabolic process; negative regulation of fibroblast growth factor receptor signaling pathway; metabolic process; cartilage development; embryonic skeletal development; glomerular filtration; esophagus smooth muscle contraction; glomerular basement membrane development; heparan sulfate proteoglycan metabolic process; chondrocyte development; kidney development; positive regulation of Wnt receptor signaling pathway
Research Articles on Sulf2
Similar Products
Product Notes
The Sulf2 sulf2 (Catalog #AAA219851) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN SULFATASE 2 (C-TERMINAL) reacts with Mouse N.B Antibody reactivity and working conditions may vary between species. and may cross-react with other species as described in the data sheet. AAA Biotech's SULFATASE 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin, Western Blot (WB). This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Researchers should empirically determine the suitability of the Sulf2 sulf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SULFATASE 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
