Rabbit anti-Human FOXC2 Polyclonal Antibody | anti-FOXC2 antibody

FOXC2, ID (S174) (FOXC2, FKHL14, MFH1, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, Transcription factor FKH-14) (MaxLight 750)

Cat. Num. AAA6299739

• Gene Names: FOXC2; LD; MFH1; MFH-1; FKHL14
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: FOXC2; Polyclonal Antibody; ID (S174) (FOXC2; FKHL14; MFH1; Forkhead box protein C2; Forkhead-related protein FKHL14; Mesenchyme fork head protein 1; Transcription factor FKH-14) (MaxLight 750); Transcription factor FKH-14; anti-FOXC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FOXC2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-FOXC2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 159-186 in the central region of human FOXC2.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A2058 cell line lysates (15ug/lane) usingMBS649063. This demonstrates that MBS649063 detected the FOXC2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma using MBS649063 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649063 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of A2058 cells using MBS649063 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FOXC2 antibody

Antibodies; Transcription Factors

References

1. van Dongen, M.J., et al. J. Mol. Biol. 296(2):351-359(2000). 2. Mangion, J., et al. Am. J. Hum. Genet. 65(2):427-432(1999). 3. Miura, N., et al. Genomics 41(3):489-492(1997). 4. Kaestner, K.H., et al. Development 122(6):1751-1758(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4885237
• NCBI GeneID: 2303
• NCBI Accession #: NP_005242
• NCBI GenBank Nucleotide #: NM_005251
• UniProt Accession #: Q99958
• Molecular Weight: 54kDa
• NCBI Official Full Name: forkhead box protein C2
• NCBI Official Synonym Full Names: forkhead box C2
• NCBI Official Symbol: FOXC2
• NCBI Official Synonym Symbols: LD; MFH1; MFH-1; FKHL14
• NCBI Protein Information: forkhead box protein C2
• UniProt Protein Name: Forkhead box protein C2
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXC2
• UniProt Synonym Gene Names: FKHL14; MFH1; MFH-1 protein
• UniProt Entry Name: FOXC2_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC2: Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2); also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment. Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN). LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities. Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD). LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).

Protein type: Cell development/differentiation; Transcription factor; Apoptosis; DNA-binding

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; neural crest cell development; heart development; positive regulation of transcription, DNA-dependent; response to hormone stimulus; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ureteric bud development; lymphangiogenesis; ventricular cardiac muscle morphogenesis; mesoderm development; regulation of blood vessel size; somitogenesis; Notch signaling pathway; ossification; camera-type eye development; positive regulation of cell adhesion mediated by integrin; regulation of organ growth; embryonic viscerocranium morphogenesis; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; paraxial mesodermal cell fate commitment; insulin receptor signaling pathway; artery morphogenesis; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; vascular endothelial growth factor receptor signaling pathway; metanephros development

Disease: Lymphedema-distichiasis Syndrome