Porcine Angiotensin 2 Receptor 1 Antibody ELISA Kit | AT2R1 elisa kit

Porcine Angiotensin 2 Receptor 1 Antibody ELISA Kit

Cat. Num. AAA743547

• Gene Names: AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
• Reactivity: Porcine
• Synonyms: Angiotensin 2 Receptor 1 Antibody; Porcine Angiotensin 2 Receptor 1 Antibody ELISA Kit; AT2R1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This AT1R-AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AT1R-AA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AT2R1 elisa kit

Principle of the Assay: AT1R-AA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT1R-AA antibody and an AT1R-AA-HRP conjugate. The assay sample and buffer are incubated together with AT1R-AA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT1R-AA concentration since AT1R-AA from samples and AT1R-AA-HRP conjugate compete for the anti-AT1R-AA antibody binding site. Since the number of sites is limited, as more sites are occupied by AT1R-AA from the sample, fewer sites are left to bind AT1R-AA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT1R-AA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AT2R1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 46250427
• NCBI GeneID: 185
• Molecular Weight: 41,061 Da
• NCBI Official Full Name: Angiotensin II receptor, type 1
• NCBI Official Synonym Full Names: angiotensin II receptor, type 1
• NCBI Official Symbol: AGTR1
• NCBI Official Synonym Symbols: AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
• NCBI Protein Information: type-1 angiotensin II receptor; type-1B angiotensin II receptor
• UniProt Protein Name: Type-1 angiotensin II receptor
• UniProt Gene Name: AGTR1
• UniProt Synonym Gene Names: AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1
• UniProt Entry Name: AGTR1_HUMAN

NCBI Description

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

AT1: receptor for angiotensin II. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. Mediates the major cardiovascular effects of angiotensin II. May play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; angiotensin type II receptor activity; protein heterodimerization activity; angiotensin type I receptor activity; bradykinin receptor binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of vasodilation; positive regulation of cellular protein metabolic process; renin-angiotensin regulation of aldosterone production; calcium-mediated signaling; regulation of systemic arterial blood pressure by renin-angiotensin; Rho protein signal transduction; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; renin-angiotensin regulation of blood vessel size; regulation of vasoconstriction; regulation of inflammatory response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of cell growth; kidney development; positive regulation of phospholipase A2 activity; positive regulation of NAD(P)H oxidase activity; positive regulation of inflammatory response

Disease: Renal Tubular Dysgenesis; Hypertension, Essential