Western Blot (WB)
(FOLR4 Antibody (N-term) (MBS9212153) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FOLR4 antibody detected the FOLR4 protein (arrow).)
Rabbit anti-Human FOLR4 Polyclonal Antibody | anti-IZUMO1R antibody
FOLR4 Antibody (N-term)
Gene Names
IZUMO1R; JUNO; FOLR4; Folbp3
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
FOLR4; Polyclonal Antibody; FOLR4 Antibody (N-term); Sperm-egg fusion protein Juno; Folate receptor 4; Folate receptor delta; FR-delta; IZUMO1 receptor protein JUNO; IZUMO1R HGNC:32565); JUNO; anti-IZUMO1R antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FOLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human FOLR4.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
17-47
Sequence Length
243
Applicable Applications for anti-IZUMO1R antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Function
Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction does not act as a membrane 'fusogen'. Does not bind folate.
Cellular Location
Cell membrane{ECO:0000250|UniProtKB:Q9EQF4};Lipid-anchor, GPI-anchor{ECO:0000250|UniProtKB:Q9EQF4}. Note=GPI-anchored at the oolemma{ECO:0000250|UniProtKB:Q9EQF4}
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(FOLR4 Antibody (N-term) (MBS9212153) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FOLR4 antibody detected the FOLR4 protein (arrow).)
Western Blot (WB)
(FOLR4 Antibody (N-term) (MBS9212153) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FOLR4 antibody detected the FOLR4 protein (arrow).)
Western Blot (WB)
(All lanes: FOLR4 Antibody (N-term) at 1:500 dilution Lane 1: HepG2 whole cell lysateLane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes: FOLR4 Antibody (N-term) at 1:500 dilution Lane 1: HepG2 whole cell lysateLane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-IZUMO1R antibody
Folate receptor 4 is a highly expressed cell surface marker of regulatory T cells.
Product Categories/Family for anti-IZUMO1R antibody
References
Spiegelstein, O., et al. Gene 258 (1-2), 117-125 (2000) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sperm-egg fusion protein Juno
NCBI Official Synonym Full Names
IZUMO1 receptor, JUNO
NCBI Official Symbol
IZUMO1R
NCBI Official Synonym Symbols
JUNO; FOLR4; Folbp3
NCBI Protein Information
sperm-egg fusion protein Juno
UniProt Protein Name
Sperm-egg fusion protein Juno
Protein Family
UniProt Gene Name
IZUMO1R
UniProt Synonym Gene Names
FR-delta
UniProt Entry Name
JUNO_HUMAN
Uniprot Description
FOLR4: Belongs to the folate receptor family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11q21
Cellular Component: plasma membrane
Molecular Function: protein binding; receptor activity; folic acid binding
Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; single fertilization; cell adhesion
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Product Notes
The IZUMO1R izumo1r (Catalog #AAA9212153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 17-47. The FOLR4 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOLR4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the IZUMO1R izumo1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOLR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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