ALDH1A3 recombinant protein

ALDH1A3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120891

• Gene Names: ALDH1A3; ALDH6; RALDH3; ALDH1A6
• Purity: >96% as determined by SDS-PAGE
• Synonyms: ALDH1A3; ALDH1A3 Protein; Human; Recombinant (His Tag); Human ALDH1A3/RALDH3 Protein (His Tag); ALDH1A6 Protein; ALDH6 Protein; MCOP8 Protein; RALDH3 Protein; aldehyde dehydrogenase 1 family; member A3; ALDH1A3 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Liquid. Supplied as sterile PBS 7.4 10% glycerol 0.2% TWEEN20. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Pro512

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ALDH1A3 (NP_000684.2)(Ala 2-Pro 512) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; It is shipped out with blue ice.

SDS-Page

Related Product Information for ALDH1A3 recombinant protein

Background: Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3), also known as Retinaldehyde dehydrogenase 3 (RALDH3), which belongs to the aldehyde dehydrogenase family. ALDH1A3 is a novel Cancer stem cell (CSC) marker with potential clinical prognostic applicability, and demonstrates a clear correlation between CSC prevalence and the development of metastatic breast cancer. The retinoic acid (RA) biosynthesis enzyme aldehyde dehydrogenase 1A3 (ALDH1A3) is a putative androgen-responsive gene in human prostate cancer epithelial (LNCaP) cells. The RA biosynthesis enzyme ALDH1A3 is androgen responsive and (ii) DHT up-regulation of ALDH1A3 can increase the oxidation of retinal to RA and indirectly affect RA bioactivity and metabolism.

References

Marcato P, et al. (2011) Aldehyde dehydrogenase activity of breast cancer stem cells is primarily due to isoform ALDH1A3 and its expression is predictive of metastasis. Stem Cells. 29(1)32-45.Trasino SE, et al. (2007) Androgen regulation of aldehyde dehydrogenase 1A3 (ALDH1A3) in the androgen-responsive human prostate cancer cell line LNCaP. Exp Biol Med (Maywood). 232(6): 762-71.

NCBI and Uniprot Product Information

• NCBI GI #: 153266822
• NCBI GeneID: 220
• NCBI Accession #: NP_000684.2
• NCBI GenBank Nucleotide #: NM_000693.2
• UniProt Accession #: P47895; Q6NT64; Q7Z3A2
• Molecular Weight: 56,108 Da
• NCBI Official Full Name: aldehyde dehydrogenase family 1 member A3
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member A3
• NCBI Official Symbol: ALDH1A3
• NCBI Official Synonym Symbols: ALDH6; RALDH3; ALDH1A6
• NCBI Protein Information: aldehyde dehydrogenase family 1 member A3; RALDH-3; OTTHUMP00000194835; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3
• UniProt Protein Name: Aldehyde dehydrogenase family 1 member A3
• Protein Family: Aldehyde dehydrogenase family
• UniProt Gene Name: ALDH1A3
• UniProt Synonym Gene Names: ALDH6; RALDH-3; RalDH3
• UniProt Entry Name: AL1A3_HUMAN

NCBI Description

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Uniprot Description

ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family.

Protein type: Oxidoreductase; Amino Acid Metabolism - histidine; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.2.1.5; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity

Biological Process: inner ear morphogenesis; retinal metabolic process; righting reflex; embryonic eye morphogenesis; retinol metabolic process; positive regulation of apoptosis; olfactory pit development; retinoic acid metabolic process; optic cup morphogenesis involved in camera-type eye development; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development

Disease: Microphthalmia, Isolated 8