Rabbit anti-Human CD9 Polyclonal Antibody | anti-CD9 antibody

CD9 Antibody (N-term)

Cat. Num. AAA9214761

• Gene Names: CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CD9; Polyclonal Antibody; CD9 Antibody (N-term); CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; MIC3; TSPAN29; anti-CD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from the N-terminal region of human CD9.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 21-51
• Sequence Length: 228

• Applicable Applications for anti-CD9 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; FC~~1:10~50
• Clone Names: RB13422
• Function: Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion(PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility andtumor metastasis (PubMed:8478605, PubMed:7511626).
• Cellular Location: Cell membrane; Multi-pass membrane protein Membrane; Multi-pass membrane protein. Secreted, exosome {ECO:0000250|UniProtKB:P40240}. Note=Present at the cell surface of oocytes. Accumulates in the adhesion area between the sperm and egg following interaction between IZUMO1 and its receptor IZUMO1R/JUNO. {ECO:0000250|UniProtKB:P40240}
• Tissue Location: Detected in platelets (at protein level) (PubMed:19640571). Expressed by a variety of hematopoietic and epithelial cells (PubMed:19640571).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CD9 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using CD9 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CD9 antibody

CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.

Product Categories/Family for anti-CD9 antibody

Cardiovascular; Developmental Biology; Metabolism; Signal Transduction

References

Ovalle,S., Int. J. Cancer 121 (10), 2140-2152 (2007)
Kovalenko,O.V., Mol. Cell Proteomics 6 (11), 1855-1867 (2007)
Abache,T., J. Cell. Biochem. 102 (3), 650-664 (2007)
Horejsi,V., FEBS Lett. 288 (1-2), 1-4 (1991)

NCBI and Uniprot Product Information

• NCBI GI #: 4502693
• NCBI GeneID: 928
• NCBI Accession #: NP_001760.1
• NCBI GenBank Nucleotide #: NM_001769.3
• UniProt Accession #: P21926; Q5J7W6; Q96ES4; D3DUQ9
• NCBI Official Full Name: CD9 antigen
• NCBI Official Synonym Full Names: CD9 molecule
• NCBI Official Symbol: CD9
• NCBI Official Synonym Symbols: MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• NCBI Protein Information: CD9 antigen
• UniProt Protein Name: CD9 antigen
• Protein Family: CD9 antigen
• UniProt Gene Name: CD9
• UniProt Synonym Gene Names: MIC3; TSPAN29; MRP-1; Tspan-29
• UniProt Entry Name: CD9_HUMAN

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Uniprot Description

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Cell adhesion

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: negative regulation of cell proliferation; platelet activation; platelet degranulation; fusion of sperm to egg plasma membrane; single fertilization; pathogenesis; brain development; blood coagulation; oligodendrocyte development; cell motility; cell adhesion; response to water deprivation; multicellular organism reproduction; paranodal junction assembly