Rat anti-Mouse CD19 Monoclonal Antibody | anti-CD19 antibody

Anti-Ms CD19 PE

Cat. Num. AAA1801920

• Gene Names: Cd19; AW495831
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD19; Monoclonal Antibody; Anti-Ms CD19 PE; B4; Leu-12; Cvid; CD19 molecule; anti-CD19 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone Number: 1D3
• Specificity: The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD19 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-5ug/ml
• Conjugation: PE
• Immunogen: Mouse CD19-transfected cell line
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of murine peritoneal fluid cells stained using anti-mouse CD19 (1D3) PE antibody (concentration in sample 1 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of murine CD19 positive lymphocytes (red-filled) from CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-mouse CD19 (1D3) PE antibody (concentration in sample 1 ug/ml).)

Related Product Information for anti-CD19 antibody

CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

References

*Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15;171(8):4062-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/14530327ordin,*Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/11781242ordin,*Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/8566073ordina,*Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens. J Immunol. 2001 Jul 1;167(1):163-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/11418645ordin,*Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1;157(1):48-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/8683154ordina

NCBI and Uniprot Product Information

• NCBI GI #: 146149173
• NCBI GeneID: 12478
• NCBI Accession #: NP_033974.2
• NCBI GenBank Nucleotide #: NM_009844.2
• UniProt Accession #: P25918; Q542B2
• Molecular Weight: 60,149 Da
• NCBI Official Full Name: B-lymphocyte antigen CD19
• NCBI Official Synonym Full Names: CD19 antigen
• NCBI Official Symbol: Cd19
• NCBI Official Synonym Symbols: AW495831
• NCBI Protein Information: B-lymphocyte antigen CD19; differentiation antigen CD19
• UniProt Protein Name: B-lymphocyte antigen CD19
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: Cd19
• UniProt Entry Name: CD19_MOUSE