Rabbit FIR Polyclonal Antibody | anti-FIR antibody

FIR (P364) Polyclonal Antibody

Cat. Num. AAA3001835

• Gene Names: FARP2; FIR; FRG; PLEKHC3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FIR; Polyclonal Antibody; FIR (P364) Polyclonal Antibody; FERM; RhoGEF and pleckstrin domain-containing protein 2; FERM domain including RhoGEF; Pleckstrin homology domain-containing family C member 3; PH domain-containing family C member 3; FARP2; KIAA0793; PLEKHC3; anti-FIR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FIR (P364) polyclonal antibody detects endogenous levels of FIR protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1054

• Applicable Applications for anti-FIR antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human FIR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FIR (P364) pAb at 1:1000 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))

Related Product Information for anti-FIR antibody

FARP2 (FERM, RhoGEF and pleckstrin domain-containing protein 2), also known as PLEKHC3 or FERM domain including RhoGEF (FIR), is a 1, 545 amino acid protein that contains one FERM domain, one DH domain and two PH domains. It exists as two alternatively spliced isoforms that are abundantly expressed in brain, lung, and testis as well as in embryonic hippocampal and cortical neurons. FARP2 functions as a Rho-guanine nucleotide exchange factor that activates RAC1 and is thought to regulate neurite remodeling of embryonic neurons. Sema3A binding to neuropilin-1 induces the dissociation of FARP2 from plexin-A1, thereby activating FARP2's Rac GEF activity which is critical for repulsion of outgrowing axons and suppression of neuronal adhesion. Downregulation of the FARP2 gene has been implicated in autism.

NCBI and Uniprot Product Information

• NCBI GI #: 7662310
• NCBI GeneID: 9855
• NCBI Accession #: NP_055623.1
• NCBI GenBank Nucleotide #: NP_055623.1
• UniProt Accession #: O94887
• Molecular Weight: ~ 120kDa
• NCBI Official Full Name: FERM, ARHGEF and pleckstrin domain-containing protein 2 isoform a
• NCBI Official Synonym Full Names: FERM, ARH/RhoGEF and pleckstrin domain protein 2
• NCBI Official Symbol: FARP2
• NCBI Official Synonym Symbols: FIR; FRG; PLEKHC3
• NCBI Protein Information: FERM, ARHGEF and pleckstrin domain-containing protein 2
• UniProt Protein Name: FERM, RhoGEF and pleckstrin domain-containing protein 2
• UniProt Gene Name: FARP2
• UniProt Synonym Gene Names: KIAA0793; PLEKHC3; FIR
• UniProt Entry Name: FARP2_HUMAN

Uniprot Description

FARP2: Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoskeleton; extrinsic to membrane; cytoplasm; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity; cytoskeletal protein binding

Biological Process: axon guidance; regulation of integrin activation; actin cytoskeleton reorganization; Rac protein signal transduction; osteoclast differentiation; cell adhesion; neuron remodeling