Rabbit ILKAP Polyclonal Antibody | anti-ILKAP antibody

ILKAP (K71) Polyclonal Antibody

Cat. Num. AAA3002499

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ILKAP; Polyclonal Antibody; ILKAP (K71) Polyclonal Antibody; Integrin-linked kinase-associated serine/threonine phosphatase 2C; anti-ILKAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LKAP (K71)pAb detects endogenous levels of ILKAP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 392

• Applicable Applications for anti-ILKAP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 40-90 of Human ILKAP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ILKAP (K71) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:H9C2 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ILKAP (K71) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of ILKAP (K71) pAb at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug))

Related Product Information for anti-ILKAP antibody

ILKAP (integrin-linked kinase-associated serine/threonine phosphatase 2C), also known as PP2Cdelta, is a 392 amino acid cytoplasmic protein phosphatase that selectively interacts with integrin linked kinase (ILK) to regulate growth factor signaling and cell adhesion. While widely expressed, ILKAP is found at highest levels in striated muscle with lower levels found in smooth muscle.ILKAP belongs to the PP2C family and contains one PP2C-like domain. ILKAP has been suggested to inhibit oncogenic transformation and the ILK-GSK3betasignaling axis, and can bind two magnesium or manganese ions per subunit as cofactors. The gene encoding ILKAP maps to human chromosome 2, which consists of 237 million bases encoding over 1, 400 genes and making up approximately 8% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 13540531
• NCBI Accession #: NP_110395.1
• NCBI GenBank Nucleotide #: NP_110395.1
• UniProt Accession #: Q9H0C8
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: integrin-linked kinase-associated serine/threonine phosphatase 2C
• UniProt Protein Name: Integrin-linked kinase-associated serine/threonine phosphatase 2C
• Protein Family: Integrin-linked kinase-associated serine/threonine phosphatase
• UniProt Gene Name: ILKAP
• UniProt Synonym Gene Names: ILKAP
• UniProt Entry Name: ILKAP_HUMAN

Uniprot Description

ILKAP: a protein serine/threonine phosphatase of the PP2C family. Can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; regulation of DNA replication during S phase