Rabbit anti-Human Natriuretic Peptide Receptor A/GC-A/NPR1 Polyclonal Antibody | anti-NPR1 antibody

Anti-Natriuretic Peptide Receptor A/GC-A/NPR1 Antibody

Cat. Num. AAA1752961

• Gene Names: NPR1; ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Natriuretic Peptide Receptor A/GC-A/NPR1; Polyclonal Antibody; Anti-Natriuretic Peptide Receptor A/GC-A/NPR1 Antibody; Atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor type A; ANP-A; ANPR-A; NPR-A; Guanylate cyclase A; GC-A; NPR1; ANPRA; anti-NPR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1061

• Applicable Applications for anti-NPR1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml (Human); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human Natriuretic Peptide Receptor A/GC-A/NPR1 recombinant protein (Position: A58-K501).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunofluorescence (IF)

Western Blot (WB)

(Figure 1. Western blot analysis of NPR1 using anti-NPR1 antibody.)

Related Product Information for anti-NPR1 antibody

Description: Rabbit IgG polyclonal antibody for Natriuretic Peptide Receptor A/GC-A/NPR1 detection. Tested with WB, ICC/IF, Direct ELISA in Human.

Background: Natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A), also known as NPR1, is an atrial natriuretic peptide receptor. In humans it is encoded by the NPR1 gene. It is mapped to 1q21.3. Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms. The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B. Also see NPR3, which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides and BNP.

References

1. Ferrari, P., Weidmann, P., Ferrier, C., Dietler, R., Hollmann, R., Piso, R. J., Wey, J., Shaw, S. Dysregulation of atrial natriuretic factor in hypertension-prone man. J. Clin. Endocr. Metab. 71: 944-951, 1990.
2. Garbers, D. L., Lowe, D. G. Guanylyl cyclase receptors. J. Biol. Chem. 269: 30741-30744, 1994.
3. Kishimoto, I., Rossi, K., Garbers, D. L. A genetic model provides evidence that the receptor for atrial natriuretic peptide (guanylyl cyclase-A) inhibits cardiac ventricular myocyte hypertrophy. Proc. Nat. Acad. Sci. 98: 2703-2706, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 167830411
• NCBI GeneID: 4881
• NCBI Accession #: NP_000897.3
• NCBI GenBank Nucleotide #: NP_000897.3
• UniProt Accession #: P16066
• NCBI Official Full Name: atrial natriuretic peptide receptor 1
• NCBI Official Synonym Full Names: natriuretic peptide receptor 1
• NCBI Official Symbol: NPR1
• NCBI Official Synonym Symbols: ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• NCBI Protein Information: atrial natriuretic peptide receptor 1
• UniProt Protein Name: Atrial natriuretic peptide receptor 1
• Protein Family: Nitrogen permease reactivator protein
• UniProt Gene Name: NPR1
• UniProt Synonym Gene Names: ANPRA; ANP-A; ANPR-A; NPR-A; GC-A

NCBI Description

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]

Uniprot Description

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.