Rabbit Neuropilin-2 Polyclonal Antibody | anti-M373 antibody

Neuropilin-2 (M373) Polyclonal Antibody

Cat. Num. AAA3004146

• Gene Names: NRP2; NP2; NPN2; PRO2714; VEGF165R2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Neuropilin-2; Polyclonal Antibody; Neuropilin-2 (M373) Polyclonal Antibody; Vascular endothelial cell growth factor 165 receptor 2; VEGF165R2; NRP2; anti-M373 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Neuropilin-2 (M373) polyclonal antibody detects endogenous levels of Neuropilin-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 931

• Applicable Applications for anti-M373 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human Neuropilin-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Neuropilin-2 (M373) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:A549 whole cell lysate (20ug) Lane4:PC12 whole cell lysate (40ug) Lane5:The Kidney tissue lysate of Mouse (40ug))

Related Product Information for anti-M373 antibody

Neuropilin is a type I transmembrane receptor that has been implicated in aspects of axon growth and guidance and has been shown to act as a high affinity receptor for class III semaphorins and vascular endothelial growth factor (VEGF). A closely related protein, neuropilin-2, shares a common domain structure and signifcant homology with neuropilin and also acts as a receptor for the class III semaphorins and VEGF. Both neuropilins are involved in regulating many physiological pathways including axonal guidance and angiogenesis, however they exhibit differential expression in the adult vasculature. Neuropilin-2 is polysialylated and expressed on the surface of dendritic cells. It is also expressed by venous and lymphatic endothelium. Neuropilin is expressed predominantly by arterial endothelium.

NCBI and Uniprot Product Information

• NCBI GI #: 2978562
• NCBI GeneID: 8828
• UniProt Accession #: O60462
• Molecular Weight: ~92kDa
• NCBI Official Full Name: vascular endothelial cell growth factor 165 receptor 2
• NCBI Official Synonym Full Names: neuropilin 2
• NCBI Official Symbol: NRP2
• NCBI Official Synonym Symbols: NP2; NPN2; PRO2714; VEGF165R2
• NCBI Protein Information: neuropilin-2
• UniProt Protein Name: Neuropilin-2
• Protein Family: Neuropilin
• UniProt Gene Name: NRP2
• UniProt Synonym Gene Names: VEGF165R2
• UniProt Entry Name: NRP2_HUMAN

NCBI Description

This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NRP2: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. Heterodimer with NRP1. Binds PLXNB1. Belongs to the neuropilin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; semaphorin receptor activity; cytokine binding; growth factor binding; metal ion binding; receptor activity

Biological Process: axon extension involved in axon guidance; axon guidance; positive regulation of endothelial cell proliferation; nerve development; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell adhesion