Mouse anti-Human FHOD1 Polyclonal Antibody | anti-FHOD1 antibody

FHOD1 (FH1/FH2 Domain-containing Protein 1, Formin Homolog Overexpressed in Spleen 1, FHOS, Formin Homology 2 Domain-containing Protein 1, FHOS, FHOS1)

Cat. Num. AAA6010437

• Gene Names: FHOD1; FHOS
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FHOD1; Polyclonal Antibody; FHOD1 (FH1/FH2 Domain-containing Protein 1; Formin Homolog Overexpressed in Spleen 1; FHOS; Formin Homology 2 Domain-containing Protein 1; FHOS1); Anti -FHOD1 (FH1/FH2 Domain-containing Protein 1; anti-FHOD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FHOD1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAGGEDRGDGEPVSVVTVRVQYLEDTDPFACANFPEPRRAPTCSLDGALPLGAQIPAVHRLLGAPLKLEDCALQVSPSGYYLDTELSLEEQREMLEGFYEEISKGRKPTLILRTQLSVRVNAILEKLYSSSGPELRRSLFSLKQIFQEDKDLVPEFVHSEGLSCLIRVGAAADHNYQSYILRALGQLMLFVDGMLGVVAHSDTIQWLYTLCASLSRLVVKTALKLLLVFVEYSENNAPLFIRAVNSVASTTGAPPWANLVSILEEKNGADPELLVYTVTLINKTLAALPDQDSFYDVTDALEQQGMEALVQRHLGTAGTDVDLRTQLVLYENALKLEDGDIEEAPGAGGRRERRKPSSEEGKRSRRSLEGGGCPARAPEPGPTGPASPVGPTSSTGPALLTGPASSPVGPPSGLQASVNLFPTISVAPSADTSSERSIYKARFLENVAAAETEKQVALAQGRAETLAGAMPNEAGGHPDARQLWDSPETAPAARTPQSPAPCVLLRAQRSLAPEPKEPLIPASPKAEPIWELPTRAPRLSIGDLDFSDLGEDEDQDMLNVESVEAGKDIPAPSPPLPLLSGVPPPPPLPPPPPIKGPFPPPPPLPLAAPLPHSVPDSSALPTKRKTVKLFWRELKLAGGHGVSASRFGPCATLWASLDPVSVDTARLEHLFESRAKEVLPSKKAGEGRRTMTTVLDPKRSNAINIGLTTLPPVHVIKAALLNFDEFAVSKDGIEKLLTMMPTEEERQKIEEAQLANPDIPLGPAENFLMTLASIGGLAARLQLWAFKLDYDSMEREIAEPLFDLKVGMEQLVQNATFRCILATLLAVGNFLNGSQSSGFELSYLEKVSEVKDTVRRQSLLHHLCSLVLQTRPESSDLYSEIPALTRCAKVDFEQLTENLGQLERRSRAAEESLRSLAKHELAPALRARLTHFLDQCARRVAMLRIVHRRVCNRFHAFLLYLGYTPQAAREVRIMQFCHTLREFALEYRTCRERVLQQQQKQATYRERNKTRGRMITETEKFSGVAGEAPSNPSVPVAVSSGPGRGDADSHASMKSLLTSRPEDTTHNRRSRGMVQSSSPIMPTVGPSTASPEEPPGSSLPSDTSDEIMDLLVQSVTKSSPRALAARERKRSRGNRKSLRRTLKSGLGDDLVQALGLSKGPGLEV

• Applicable Applications for anti-FHOD1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
• Immunogen: Full length human FHOD1, aa1-1164 (NP_037373.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FHOD1 polyclonal antibody. Western Blot analysis of FHOD1 expression in HepG2.)

Western Blot (WB)

(FHOD1 polyclonal antibody. Western Blot analysis of FHOD1 expression in K-562.)

Western Blot (WB)

(Western Blot analysis of FHOD1 expression in transfected 293T cell line by FHOD1 polyclonal antibody. Lane 1: FHOD1 transfected lysate (128.04kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of purified antibody to FHOD1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml])

Related Product Information for anti-FHOD1 antibody

FHOD1 is a member of the formin homology (FH) family of proteins bearing significant homology to Formins and Diaphanous proteins in the FH1 and FH2 domains. FHOD1 contains all the conserved domains, including FH1, FH2, and FH3 domains, and a coiled-coil, characteristic of FH protein. It also contains two nuclear localization signals, a classic lysine:arginine-rich domain and a consensus bipartite signal. FHOD1 induces transcription from serum response factor by multiple pathways. It interacts with Rac1 GTPase and induces the formation of and associates with bundled actin stress fibers. The protein also coordinates microtubules with these actin stress fibers thus mediating cell elongation. RNA blot analysis revealed high levels of FHOD1 mRNA in the spleen, skeletal muscle, and lung. Human FHOD1 gene is localized in the chromosomal region 16q22.

Product Categories/Family for anti-FHOD1 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 118572599
• NCBI GeneID: 29109
• NCBI Accession #: NP_037373.2
• NCBI GenBank Nucleotide #: NM_013241.2
• UniProt Accession #: Q9Y613; Q59F76; Q6Y1F2; Q76MS8; Q8N521
• Molecular Weight: 126,551 Da
• NCBI Official Full Name: FH1/FH2 domain-containing protein 1
• NCBI Official Synonym Full Names: formin homology 2 domain containing 1
• NCBI Official Symbol: FHOD1
• NCBI Official Synonym Symbols: FHOS
• NCBI Protein Information: FH1/FH2 domain-containing protein 1; formin homolog overexpressed in spleen 1
• UniProt Protein Name: FH1/FH2 domain-containing protein 1
• Protein Family: FH1/FH2 domain-containing protein
• UniProt Gene Name: FHOD1
• UniProt Synonym Gene Names: FHOS; FHOS1; FHOS
• UniProt Entry Name: FHOD1_HUMAN

NCBI Description

This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. [provided by RefSeq, Jul 2008]

Uniprot Description

FHOD1: a cytoskeletal protein required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C- terminus.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: membrane; cytoplasm; stress fiber; nucleus

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter