Chemiluminescence
((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FEM1A Polyclonal Antibody
FEM1A (Protein Fem-1 Homolog A, FEM1a, FEM1-alpha, Prostaglandin E Receptor 4-associated Protein, EPRAP)
Reactivity
Bovine, Canine, Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
FEM1A; Polyclonal Antibody; FEM1A (Protein Fem-1 Homolog A; FEM1a; FEM1-alpha; Prostaglandin E Receptor 4-associated Protein; EPRAP); Anti -FEM1A (Protein Fem-1 Homolog A; anti-FEM1A antibody
Host
Goat
Reactivity
Bovine, Canine, Human
Clonality
Polyclonal
Specificity
Recognizes human FEM1A. Species sequence homology: Bovine and canine.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-FEM1A antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:4000
Western Blot: 1-3ug/ml, observed in human heart lysates on ~73.6kD bands
Dilution: ELISA: 1:4000
Western Blot: 1-3ug/ml, observed in human heart lysates on ~73.6kD bands
Immunogen
Synthetic peptide corresponding to C-GFIPEDLEAFIELH, from human FEM1A, at C-terminal (NP_061178.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-FEM1A antibody
Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4.
Product Categories/Family for anti-FEM1A antibody
NCBI and Uniprot Product Information
Uniprot Description
FEM1C: Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Belongs to the fem-1 family.
Protein type: Ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q22
Cellular Component: cytoplasm
Biological Process: protein ubiquitination
Similar Products
Product Notes
The FEM1A (Catalog #AAA6003975) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The FEM1A (Protein Fem-1 Homolog A, FEM1a, FEM1-alpha, Prostaglandin E Receptor 4-associated Protein, EPRAP) reacts with Bovine, Canine, Human and may cross-react with other species as described in the data sheet. AAA Biotech's FEM1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:4000 Western Blot: 1-3ug/ml, observed in human heart lysates on ~73.6kD bands. Researchers should empirically determine the suitability of the FEM1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FEM1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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