Goat FEM1A/APRAP Polyclonal Antibody | anti-FEM1A antibody

Goat anti-FEM1A/APRAP Antibody

Cat. Num. AAA422098

• Gene Names: FEM1A; EPRAP
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FEM1A/APRAP; Polyclonal Antibody; Goat anti-FEM1A/APRAP Antibody; FEM1A; fem-1 homolog a (C. elegans); EPRAP; fem-1 homolog a; prostaglandin E receptor 4-associated protein; DKFZp762M136; FEM1A antibody; fem-1 homolog a (C. elegans) antibody; EPRAP antibody; fem-1 homolog a antibody; prostaglandin E receptor 4-associated protein antibody; DKFZp762M136 antibody; FEM1A / APRAP; anti-FEM1A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: GFIPEDLEAFIELH
• Sequence Length: 669

• Applicable Applications for anti-FEM1A antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Approx.73.6kDa band observed in Human Heart lysates (calculated MW of 73.6kDa according to NP_061178.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-GFIPEDLEAFIELH, from the C Terminus of the protein sequence according to NP_061178.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 24308163
• NCBI GeneID: 55527
• NCBI Accession #: NP_061178.1
• NCBI GenBank Nucleotide #: NM_018708.2
• Molecular Weight: 73,639 Da
• NCBI Official Full Name: protein fem-1 homolog A
• NCBI Official Synonym Full Names: fem-1 homolog A
• NCBI Official Symbol: FEM1A
• NCBI Official Synonym Symbols: EPRAP
• NCBI Protein Information: protein fem-1 homolog A
• UniProt Protein Name: Protein fem-1 homolog A
• Protein Family: Protein
• UniProt Gene Name: FEM1A
• UniProt Synonym Gene Names: EPRAP; FEM1a
• UniProt Entry Name: FEM1A_HUMAN

Uniprot Description

FEM1A: Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4. Belongs to the fem-1 family.

Protein type: Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm

Molecular Function: EP4 subtype prostaglandin E2 receptor binding; ubiquitin-protein ligase activity

Biological Process: negative regulation of inflammatory response; protein ubiquitination; regulation of ubiquitin-protein ligase activity