Typical Testing Data/Standard Curve (for reference only)
Rat CCAAT/Enhancer Binding Protein Alpha (CEBPa) ELISA Kit | CEBPa elisa kit
Rat CCAAT/Enhancer Binding Protein Alpha (CEBPa) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CEBPa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CEBPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CEBPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CEBPa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Uniprot Description
C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: Rb-E2F complex; nuclear matrix; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; embryonic placenta development; macrophage differentiation; viral reproduction; cell maturation; response to glucocorticoid stimulus; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; response to vitamin B2; acute-phase response; inner ear development; mitochondrion organization and biogenesis; cholesterol metabolic process; organ regeneration; generation of precursor metabolites and energy; transcription, DNA-dependent; granulocyte differentiation; cytokine and chemokine mediated signaling pathway; negative regulation of cyclin-dependent protein kinase activity; liver development; positive regulation of osteoblast differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; white fat cell differentiation; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; urea cycle; lung development
Disease: Leukemia, Acute Myeloid
Research Articles on CEBPa
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Product Notes
The Rat CEBPa cebpa (Catalog #AAA2025130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2025130 ELISA Kit recognizes Rat CEBPa. It is sometimes possible for the material contained within the vial of "CCAAT/Enhancer Binding Protein Alpha (CEBPa), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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