Rabbit FCER1G Polyclonal Antibody | anti-FCER1G antibody

FCER1G Antibody

Cat. Num. AAA9612022

• Gene Names: FCER1G; FCRG
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FCER1G; Polyclonal Antibody; FCER1G Antibody; Fc fragment of IgE; high affinity I; receptor for; gamma polypeptide; Fc receptor gamma chain; Fc-epsilon RI-gamma; FCERG_HUMAN; FceRI gamma; FCRG; FcRgamma; High affinity immunoglobulin epsilon receptor subunit gamma; IgE Fc receptor subunit gamma; Immunoglobulin E receptor; high affinity; gamma chain; anti-FCER1G antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FCER1G Antibody detects endogenous levels of total FCER1G.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 86

• Applicable Applications for anti-FCER1G antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human FCER1G, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells, using FCER1G. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612022 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612022 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-FCER1G antibody

Function: Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells. As a constitutive component of interleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset. Associates with pattern recognition receptors CLEC4D and CLEC4E to form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediated neutrophil activation. Also involved in integrin alpha-2/ITGA2-mediated platelet activation.

Subcellular Location: Cell membrane>Single-pass type I membrane protein.

Subunit Structure: IgE Fc receptor is a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains. Associates with FCGR1A; forms a functional signaling complex (PubMed:8611682). Associated with CLEC6A (By similarity). Interacts with CLEC4E (PubMed:18776906). Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK, enabling integrin-mediated activation of neutrophils and macrophages (By similarity). Interacts with CSF2RB and recruits SYK in response to IL3 stimulation; this interaction is direct (By similarity). Interacts with CD300LH; the interaction may be indirect (By similarity). Interacts with CD300LD (By similarity). Interacts with TARM1 (PubMed:26311901).

Similarity: Belongs to the CD3Z/FCER1G family.

NCBI and Uniprot Product Information

• NCBI GI #: 4758344
• NCBI GeneID: 2207
• NCBI Accession #: NP_004097.1
• NCBI GenBank Nucleotide #: NP_004097.1
• UniProt Accession #: P30273
• Molecular Weight: Observed Molecular Weight: (Observed)9kD.; Predicted Molecular Weight: (Calculated)10kDa.
• NCBI Official Full Name: high affinity immunoglobulin epsilon receptor subunit gamma
• NCBI Official Synonym Full Names: Fc fragment of IgE receptor Ig
• NCBI Official Symbol: FCER1G
• NCBI Official Synonym Symbols: FCRG
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit gamma
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit gamma
• Protein Family: High affinity immunoglobulin epsilon receptor
• UniProt Gene Name: FCER1G
• UniProt Synonym Gene Names: FcRgamma; FceRI gamma
• UniProt Entry Name: FCERG_HUMAN

NCBI Description

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq, Jul 2008]