Rabbit anti-Human GFAP Polyclonal Antibody | anti-GFAP antibody

Anti-GFAP Antibody, Rabbit Polyclonal

Cat. Num. AAA8100142

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: GFAP; Polyclonal Antibody; Anti-GFAP Antibody; Rabbit Polyclonal; GFAP Antibody; Rabbit PAb; Antigen Affinity Purified; glial fibrillary acidic protein; Anti-ALXDRD Antibody; anti-GFAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GFAP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GFAP antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:10,000-1:50,000
• Immunogen: A synthetic peptide corresponding to the center region of the human GFAP
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human GFAP, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GFAP in human brain with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-GFAP rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain Tissue LysateLane B: Rat brain Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:42 kDa)

Related Product Information for anti-GFAP antibody

GFAP is a cell-specific marker which belongs to the intermediate filament family. It can distinguish astrocytes from other glial cells during development. GFAP is expressed in cells lacking fibronectin. It is a type III intermediate filaments protein which contains three domains: the head, rod and tail domains. GFAP functions in many important entral nervous system (CNS) processes, including cell communication and the functioning of the blood brain barrier. Improper GFAP regulation can cause multiple disorders. Defects in GFAP is related to Alexander disease which is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes.

Product Categories/Family for anti-GFAP antibody

Neuroinflammation

References

Buniatian G, et al., 1998, Biology of the cell. 90(1): 53-61.Chen YS, et al., 2011, Experimental Cell Research. 317(16): 2252-66.Isaacs A, et al., 1998, Genomics. 51(1): 152-4.

NCBI and Uniprot Product Information

• NCBI GI #: 196115290
• NCBI GeneID: 2670
• NCBI Accession #: NP_001124491.1
• NCBI GenBank Nucleotide #: NM_001131019.2
• UniProt Accession #: P14136; Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59
• Molecular Weight: 49,880 Da
• NCBI Official Full Name: glial fibrillary acidic protein isoform 2
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease