Western Blot (WB)
(Western Blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution. Positive control: Lane1: SH-SY5Y Lane2: Human brain Lane3: Rat spleen Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen)
Rabbit Fascin Polyclonal Antibody | anti-FSCN1 antibody
Fascin Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution. Positive control: Lane1: SH-SY5Y Lane2: Human brain Lane3: Rat spleen Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen)
Western Blot (WB)
(Western Blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution. Positive control: Lane1: SH-SY5Y Lane2: Human brain Lane3: Rat spleen Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen)
Immunocytochemistry (ICC)
(ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
Uniprot Description
Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 7p22
Cellular Component: cytoplasm; stress fiber; cell junction; cytosol; actin cytoskeleton; filopodium
Molecular Function: protein binding, bridging; actin filament binding; protein binding; drug binding; actin binding
Biological Process: cell proliferation; actin filament bundle formation; cell migration; cell motility involved in cell locomotion; actin cytoskeleton organization and biogenesis
Research Articles on FSCN1
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Product Notes
The FSCN1 fscn1 (Catalog #AAA3012679) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fascin Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fascin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:1,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the FSCN1 fscn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fascin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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