Rabbit Fascin Polyclonal Antibody | anti-FSCN1 antibody

Anti-Fascin antibody

Cat. Num. AAA175408

• Gene Names: FSCN1; HSN; SNL; p55; FAN1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Fascin; Polyclonal Antibody; Anti-Fascin antibody; fascin homolog 1; actin-bundling protein(Strongylocentrotus purpuratus); 55 kDa actin bundling protein antibody; 55 kDa actin-bundling protein antibody; Actin bundling protein antibody; actin bundling protein; 55-KD antibody; FAN 1 antibody; FAN1 antibody; Fascin 1 antibody; Fascin antibody; Fascin homolog 1 actin bundling protein(Strongylocentrotus purpuratus) antibody; Fascin homolog 1 antibody; sea urchin; homolog of; 1 antibody; Fascin1 antibody; FLJ38511 antibody; FSCN 1 antibody; FSCN1 antibody; FSCN1_HUMAN antibody; HSN antibody; p55 antibody; Singed(Drosophila) like(sea urchin fascin homolog like) antibody; Singed drosophila homolog like antibody; Singed like(fascin homolog sea urchin)(Drosophila) antibody; Singed like(fascin homolog sea urchin) antibody; Singed like protein antibody; Singed; drosophila; homolog of antibody; Singed-like protein antibody; SNL antibody; Strongylocentrotus purpuratus antibody; anti-FSCN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 493

• Applicable Applications for anti-FSCN1 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin (97-113aa DDGRWSLQSEAHRRYFG), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Fascin antibody, MBS175408, Western blottingAll lanes: Anti Fascin (MBS175408) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 55KDObserved bind size: 55KD )

Related Product Information for anti-FSCN1 antibody

Description: Rabbit IgG polyclonal antibody for Fascin(FSCN1) detection. Tested with WB in Human, Mouse, Rat.
Background: Fascin is a actin cross-linking protein. The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin(from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 2498357
• NCBI GeneID: 6624
• UniProt Accession #: Q16658; Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
• Molecular Weight: 54,530 Da
• NCBI Official Full Name: Fascin
• NCBI Official Synonym Full Names: fascin actin-bundling protein 1
• NCBI Official Symbol: FSCN1
• NCBI Official Synonym Symbols: HSN; SNL; p55; FAN1
• NCBI Protein Information: fascin; 55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein; singed-like (fascin homolog, sea urchin)
• UniProt Protein Name: Fascin
• Protein Family: Fascin
• UniProt Gene Name: FSCN1
• UniProt Synonym Gene Names: FAN1; HSN; SNL
• UniProt Entry Name: FSCN1_HUMAN

NCBI Description

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Uniprot Description

Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm; stress fiber; cell junction; cytosol; actin cytoskeleton; filopodium

Molecular Function: actin filament binding; protein binding, bridging; protein binding; drug binding; actin binding

Biological Process: cell proliferation; actin filament bundle formation; cell migration; cell motility involved in cell locomotion; actin cytoskeleton organization and biogenesis